Substituted oximes as neurokinin antagonists

ABSTRACT

Compounds within the genus represented by the structural formula I ##STR1## or a pharmaceutically acceptable salt thereof, wherein: T is substituted phenyl or substituted pyridyl; ##STR2## R 4  is methyl or ethyl; and Z is substituted piperidinyl.

BACKGROUND OF THE INVENTION

The present invention relates to a genus of substituted oximes useful as antagonists of tachykinin receptors, in particular as antagonists of the neuropeptides neurokinin-1 receptor (NK₁) and/or neurokinin-2 receptor (NK₂) and/or neurokinin-3 receptor (NK₃).

Neurokinin receptors are found in the nervous system and the circulatory system and peripheral tissues of mammals, and therefore are involved in a variety of biological processes. Neurokinin receptor antagonists are consequently expected to be useful in the treatment or prevention of various mammalian disease states, for example asthma, cough, chronic obstructive pulmonary disease (COPD), bronchospasm, emesis, neurodegenerative disease, ocular disease, inflammatory diseases such as arthritis, central nervous system conditions such as migraine and epilepsy, nociception, psychosis and various gastrointestinal disorders such as Crohn's disease.

In particular, NK₁ receptors have been reported to be involved in microvascular leakage and mucus secretion, and NK₂ receptors have been associated with smooth muscle contraction, making NK₁ and NK₂ receptor antagonists especially useful in the treatment and prevention of asthma.

Some NK₁ and NK₂ receptor antagonists have previously been disclosed: arylalkylamines were disclosed in U.S. Pat. No. 5,350,852, issued Sep. 27, 1994, and spirosubstituted azacycles were disclosed in WO 94/29309, published Dec. 22, 1994.

U.S. Pat. No. 5,696,267 discloses compounds represented by the generic structure ##STR3## or a pharmaceutically acceptable salt thereof, wherein: a is 0, 1,2 or 3;

R is H, C₁₋₆ alkyl, --OH or C₂ --C₆ hydroxyalkyl;

A is an optionally substituted oxime, hydrazone or olefin;

X is a bond, --C(O)--, --O--, --NR⁶ --, --S(O)_(e) --, --N(R⁶)C(O)--, --C(O)N(R⁶)-- --OC(O)NR⁶ --, --OC(═S)NR⁶ --, --N(R⁶)C(═S)O--, --C(═NOR¹)--, --S(O)₂ N(R⁶)--, --N(R⁶)S(O)₂ --, --N(R⁶)C(O)O--or --OC(O)--;

b, d and e are independently 0, 1 or 2;

T is H, phthalimidyl, aryl, heterocycloalkyl, heteroaryl, cycloalkyl or bridged cycloalkyl;

Q is --SR⁶, --N(R⁶)(R⁷), --OR⁶, phenyl, naphthyl or heteroaryl;

R^(6a), R^(7a), R^(8a), R^(9a), R⁶ and R⁷ are H, C₁₋₆ alkyl, C₂ --C₆ hydroxyalkyl, C₁ --C₆ alkoxy-C₁ --C₆ alkyl, phenyl or benzyl; or R⁶ and R⁷, together with the nitrogen to which they are attached, form a ring;

R^(9a) is R⁶ or --OR⁶ ;

Z is morpholinyl, optionally N-substituted piperazinyl, optionally ##STR4## g is 0-3 and h is 1-4, provided the sum of h and g is 1-7; wherein aryl, heterocycloalkyl, heteroaryl, cycloalkyl and bridged cycloalkyl groups are optionally substituted.

We have found that certain compounds within that generic structure show surprisingly greater activity as neurokinin antagonists than those previously specifically disclosed.

SUMMARY OF THE INVENTION

Compounds of the present invention are represented by the formula I ##STR5## or a pharmaceutically acceptable salt thereof, wherein: T is R² -phenyl or R³ -pyridyl;

R¹ is H, methyl, ethyl, --CH₂ CN, --CH₂ C(O)NH₂, --(CH₂)₃ SO₃ H, --CH₂ C(O)NHCH₃, --CH₂ C(O)NHOH, --CH₂ C(O)NHOCH₃, --CH₂ C(O)NHCH₂ CN, --CH₂ F, --CH₂ C(O)NHCH₂ SO₃ H, ##STR6## R² is 2-3 substituents independently selected from the group consisting of chloro, methyl and methoxy;

R³ is 2 to 3 substituents independently selected from the group consisting of chloro and methyl;

R⁴ is methyl or ethyl; and

Z is ##STR7##

Preferred are Z-isomers of compounds of formula I.

Preferred are compounds of formula I wherein T is R² --phenyl, with compounds wherein R² is two chloro substituents, two methyl substituents (preferably 3,5-dichloro or 3,5-dimethyl), or two methoxy and one methyl substituent (i.e., 3,5-methoxy4-methyl) being more preferred; compounds wherein R² is two chloro groups are especially preferred.

Also preferred are compounds of formula I wherein R¹ is methyl, --CH₂ F, --CH₂ CN, --(CH₂)₃ SO₃ H, ##STR8## with methyl being more preferred.

R⁴ is preferably methyl.

Another group of preferred compounds is that wherein Z is, ##STR9## preferred, and especially preferred.

This invention also relates to the use of a compound of formula I in the treatment of asthma, cough, chronic obstructive pulmonary disease (COPD), bronchospasm, emesis, neurodegenerative disease, ocular disease, inflammatory diseases such as arthritis, central nervous system conditions such as migraine and epilepsy, nociception, psychosis, and various gastrointestinal disorders such as Crohn's disease.

In another aspect, the invention relates to a pharmaceutical composition comprising a compound of formula I in a pharmaceutically acceptable carrier. The invention also relates to the use of said pharmaceutical composition in the treatment of asthma, cough, chronic obstructive pulmonary disease (COPD), bronchospasm, emesis, neurodegenerative disease, ocular disease, inflammatory diseases such as arthritis, central nervous system conditions such as migraine and epilepsy, nociception, psychosis, and various gastrointestinal disorders such as Crohn's disease.

DETAILED DESCRIPTION

In the structural formulas shown throughout the specification and claims, hydrogen atoms may be understood, e.g., the partial structure ##STR10## is the same as ##STR11## and methyl groups may appear as a line e.g., ##STR12## is the same as ##STR13## Some formulas may include a methyl group shown as a line, and the point of attachment to another atom shown as a line through which a wavy line is drawn, i.e., ##STR14##

Compounds of formula I can have at least one asymmetric carbon atom and all isomers, including diastereomers, enantiomers and rotational isomers, as well as E and Z isomers of the oxime, hydrazone and olefin groups, are contemplated as being part of this invention. The invention includes d and I isomers in both pure form and in admixture, including racemic mixtures. Isomers can be prepared using conventional techniques, either by reacting optically pure or optically enriched starting materials or by separating isomers of a compound of formula I.

Those skilled in the art will appreciate that for some compounds of formula I, one isomer will show greater pharmacological activity than other isomers.

Compounds of the invention have at least one amino group which can form pharmaceutically acceptable salts with organic and inorganic acids. Examples of suitable acids for salt formation are hydrochloric, sulfuric, phosphoric, acetic, citric, oxalic, malonic, salicylic, malic, fumaric, succinic, ascorbic, maleic, tartaric, methanesulfonic and other mineral and carboxylic acids well known to those in the art. The salt is prepared by contacting the free base form with a sufficient amount of the desired acid to produce a salt. The free base form may be regenerated by treating the salt with a suitable dilute aqueous base solution such as dilute aqueous sodium bicarbonate. The free base form differs from its respective salt form somewhat in certain physical properties, such as solubility in polar solvents, but the salt is otherwise equivalent to its respective free base forms for purposes of the invention.

Certain compounds of the invention are acidic (e.g., those compounds which possess a carboxyl group). These compounds form pharmaceutically acceptable salts with inorganic and organic bases. Examples of such salts are the sodium, potassium, calcium, aluminum, gold and silver salts. Also included are salts formed with pharmaceutically acceptable amines such as ammonia, alkyl amines, hydroxyalkylamines, N-methylglucamine and the like.

Compounds of formula I can be prepared using methods well known to those skilled in the art, for example by procedures disclosed in WO 96/34857. The skilled artisan will recognize that other procedures may be applicable, and that the procedure may be suitably modified to prepare other compounds within the scope of formula I.

Compounds of formula I as defined above can be prepared as shown in the following reaction scheme relating to the broader scope of compounds disclosed in WO 96/34857. In the reaction scheme, the variables are as defined above for the PCT application:

Step 1: ##STR15##

In step 1, a compound of formula 47A. wherein Q is as defined above, is reacted with a base such as lithium diisopropylamide (LDA), KHMDS or KH in an inert organic solvent such as THF or DME to generate a dianion. An acid chloride, ester or amide of formula 46A, 46B, or 46C is added to give a ketone of formula 48. Preferable reaction temperatures ranges from -78° C. to 30° C.

Alternatively, compounds of formula 48 can be generated by the reaction of a compound of formula 46, preferably 46C, with a metallated species of formula QCH₂ Mt where Mt is a metal, such as lithium or MgHal, wherein "Hal" is halogen. The metallated species QCH₂ Mt can be generated by conventional procedures, such as treatment compounds of formula QCH₂ Hal with Mg or by treating QCH₃ with an organolithium base.

Step 2: ##STR16##

In step 2, for compounds disclosed inthe PCT application wherein R is not hydrogen, the ketone 48 is reacted with a suitable base, such as LDA or KH in an inert organic solvent such as THF. For compounds wherein R is alkyl or hydroxyalkyl, a compound R-R¹⁷ ", wherein R¹⁷ " is leaving group such as Br, I or triflate is added. For compounds of the PCT application wherein R is OH, an appropriate oxidizing agent such as dimethyldioxirane or Davis reagent is added. Preferable reaction temperatures range from -78° to 50° C. For compounds of the present invention, corresponding to compounds of the PCT application wherein R is H, the ketone 48 is used directly in Step 3.

Step 3: ##STR17##

In step 3, ketone 49 is reacted with a base such as LDA in a solvent such as THF, then an olefin of formula 50 is added, wherein R¹⁷ " is as defined above, to give the adduct 51. Preferable reaction temperatures range from -78° C. to 60° C.

Step 4: ##STR18##

In step 4, ketone 51 is reacted with HA', wherein A' is NH--OR¹, in an organic solvent such as pyridine or ethanol at a temperature from 25° C. to 150° C. to give a compound of formula 52.

Step 5: ##STR19##

In step 5, a compound of formula 52 is oxidized by ozonolysis to give an aldehyde of formula 53. Suitable organic solvents include EtOAc, CH₃ OH, ethanol, CH₂ Cl₂ or the like. Preferable reaction temperatures are from -78 to 0° C.

Step 6: ##STR20##

In step 6, an aldehyde of formula 53 is reacted with a compound of formula Z--H, wherein Z is as defined above. The reaction is preferably carried out with a suitably substituted amine (as its acid salt e.g. HCl or maleate or as its free base) and a hydride source such as NaBH₃ CN or sodium triacetoxyborohydride in a suitable solvent (e.g. CH₃ OH, CH₃ CH₂ OH, or CF₃ CH₂ OH for NaBH₃ CN, or THF, 1,2-dichloroethane, CH₃ CN or CF₃ CH₂ OH for triacetoxyborohydride), with 3A sieves to obtain the desired product. Any suitable temperature can be used with preferable temperatures between 0° C. and 25° C.

Alternatively, a compound of formula I can be prepared from 51 by the following reaction scheme, wherein the variables are as defined for the cited PCT application: ##STR21##

Compound 51 is oxidized to a compound of formula 54 under conditions similar to those described for step 5 above. The aldehyde of formula 54 is reacted with a compound of formula Z--H in a manner similar to that described in Step 6, and the resultant ketone is then reacted with a compound of the formula HA' as described above in Step 4 to obtain the compound of formula I.

Reactive groups not involved in the above processes can be protected during the reactions with conventional protecting groups which can be removed by standard procedures after the reaction. The following Table 1 shows some typical protecting groups:

                  TABLE 1                                                          ______________________________________                                         Group to be                                                                             Group to be Protected and                                               Protected Protecting Group                                                   ______________________________________                                         --COOH   --COOalkyl, --COObenzyl, --COOphenyl, --COOallyl                         -                                                                                      #STR22##                                                                       #STR23##                                                               -                                                                                      #STR24##                                                               -                                                                                      #STR25##                                                               - --NH.sub.2                                                                           #STR26##                                                               - --OH --OCH.sub.3, --OCH.sub.2 OCH.sub.3, --OSi(CH.sub.3).sub.3,                      -                                                                              #STR27##                                                               -  --Oallyl or --OCH.sub.2 phenyl                                           ______________________________________                                    

Compounds of formula I have been found to be antagonists of NK₁ and/or NK₂ and/or NK₃ receptors, and are therefore useful in treating conditions caused or aggravated by the stimulation of said receptors.

The present invention also relates to a pharmaceutical composition comprising a compound of formula I and a pharmaceutically acceptable carrier. Compounds of this invention can be administered in conventional oral dosage forms such as capsules, tablets, powders, cachets, suspensions or solutions, or in injectable dosage forms such as solutions, suspensions, or powders for reconstitution The pharmaceutical compositions can be prepared with conventional excipients and additives, using well known pharmaceutical formulation techniques. Pharmaceutically acceptable excipients and additives include non-toxic and chemically compatibile fillers, binders, disintegrants, buffers, preservatives, anti-oxidants, lubricants, flavorings, thickeners, coloring agents, emulsifiers and the like.

The daily dose of a compound of formula I for treating asthma, cough, bronchspasm, inflammatory diseases, migraine, nociception and gastrointestinal disorders is about 0.1 mg to about 20 mg/kg of body weight per day, preferably about 0.5 to about 15 mg/kg. For an average body weight of 70 kg, the dosage range is therefore from about 1 to about 1500 mg of drug per day, preferably about 50 to about 200 mg, more preferably about 50 to about 500 mg/kg per day, given in a single dose or 2-4 divided doses. The exact dose, however, is determined by the attending clinician and is dependent on the potency of the compound administered, the age, weight, condition and response of the patient.

Following are examples of preparing starting materials and compounds of formula I. As used herein, Me is methyl, Bu is butyl, Br is bromo, Ac is acetyl, Et is ethyl and Ph is phenyl.

Preparation 1 ##STR28##

Treat a solution of (cis)-[[[(3,5-bis(trifluoromethyl)phenyl]methoxy]methyl]4-(3,4-dichlorophenyl)-4-hydroxy-4-phenyl-1-piperidinebutanol (2.0 g, 3.08 mmol) in acetone (90 mL, 0° C.) with Jones reagent (9 mL of H₂ CrO₄ in H₂ SO₄ (ca. 8 M)). Stir the light orange suspension at 0° C. for 1 h, then partition between CH₂ Cl₂ (150 mL) and saturated aqueous NaHCO₃ (150 mL). Extract the aqueous layer with CH₂ Cl₂ (3×150 mL), back extract the combined organic layers with saturated aqueous NaHCO₃ (150 mL), dry (Na₂ SO₄) and concentrate to give 1.94 g crude product. Purify by silica gel chromatography (column: 4 cm×15 cm; eluant: EtOAc:hexane: triethylamine (66:33:2)) to obtain 1.64 g (2.53 mmol, 82%) of the title compound as a colorless foam. HRMS (FAB, M+H⁺): m/e calc'd for [C₃₁ H₃₀ NO₃ Cl₂ F₆ ]⁺ : 648.1507, found 648.1496.

Preparation 2 ##STR29## Step 1:

Dissolve 4-aminomethyl-piperidine (30.00 g, 0.263 mol) in CH₃ OH (500 mL), cool to -30° C. under N₂, add di-t-butyl dicarbonate (38.23 g, 0.175 mol) in CH₃ OH (100 mL) dropwise, warm slowly to 23° C. and stir for 16 h. Concentrate, add CH₂ Cl₂ (700 mL), wash with saturated aqueous NaCl (2×200 mL), dry organic solution (MgSO₄), filter and concentrate to give 36.80 g of a 86:14 mixture of the title compound and 1,1-dimethylethyl 4-[(1,1-dimethylethyloxycarbonyl)methyl]-1-piperidinecarboxylate.

Step 2A: ##STR30##

Dissolve the product (19.64 g, 0.0916 mol, 22.84 g of the mixture) of Step 1 in dry CH₂ Cl₂ (350 mL) and cool to 0° C. under N₂. Add pyridine (10.87 g, 11.1 mL, 0.137 mol) then chlorovaleryl chloride (15.63 g, 13.0 mL, 0.101 mol), warm slowly to 23° C. and stir for 16 h. Add saturated aqueous NH₄ Cl (300 mL), separate layers and extract with CH₂ Cl₂ (2×250 mL). Dry combined organic extracts (MgSO₄), filter and concentrate. Purify by chromatography (1000 mL of flash silica gel; eluant: 1:1 EtOAc:hexane, then EtOAc). Combine appropriate fractions and concentrate to give 25.36 g (0.0762 mol, 84%) as a colorless oil. MS (Cl/CH₄): m/e 333 (M+1) ##STR31## Step 2B:

Treat the product of Step 1 in a procedure similar to that described for Step. 2A, using chlorobutryl chloride. MS (FAB): m/e 319 (M+1)

Step 3: ##STR32## Prep. 2A:

Wash NaH (3.84 g, 0.160 mol, 6.40 g of 60 wt %) with hexane (25 mL), suspend in dry THF (150 mL) and cool to 0° C. under N₂. Add the product (25.35 g, 0.0762 mol) of Step. 2A in dry THF (150 mL) dropwise. Stir at 23° C. for 30 mins, reflux for 6 h, and stir at 23° C. for 16 h. Cool to 0° C. and add water (150 mL) and 1 N HCl (150 mL). Concentrate and extract with EtOAc (3×200 mL). Wash combined organic extracts with saturated aqueous NaCl, dry (MgSO₄), filter and concentrate. Purify by chromatography (600 mL of flash silica gel; eluant: 5% CH₃ OH--CH₂ Cl₂). Combine appropriate fractions and concentrate to give 21.62 g (0.0729 mol, 96%) of the title compound as a yellow oil. MS (FAB): m/e 297 (M+1) ##STR33## Prep. 2B:

Treat the product of Step 2B in a procedure similar to that described for Prep. 2A. MS (FAB): m/e 283 (M+1). ##STR34## Prep. 2C:

Combine the product (1.50 g, 5.06 mmol) of Prep. 2A and Lawesson reagent (1.13 g, 2.78 mmol) in dry THF (20 mL) under N₂. Stir at 23° C. for 20 h. Concentrate and purify by chromatography (200 mL of flash silica gel; eluant: 1:3 EtOAc:hexane, 1:2 EtOAc:hexane, then 1:1 EtOAc:hexane). Combine appropriate fractions and concentrate to give 1.30 g (4.16 mmol, 82%) as a green oil. MS (FAB): m/e 313 (M+1). ##STR35## Prep. 2D:

Dissolve the product (2.50 g, 8.43 mmol) of Prep. 2A in dry THF (30 mL), add borane-DMS (16.9 mL of 2.0 M in THF, 33.74 mmol) and reflux for 20 h. Cool to 0° C. and add CH₃ OH (20 mL). Concentrate, add EtOH (50 mL) and K₂ CO₃ (4.66 g, 33.74 mmol). Reflux for 4 h and cool to 23° C. Add water (100 mL), concentrate and extract with CH₂ Cl₂ (4×50 mL). Dry combined organic extracts (MgSO₄), filter and concentrate. Purify by chromatography (200 mL of flash silica gel; eluant: 7% CH₃ OH--CH₂ Cl₂). Combine appropriate fractions and concentrate to give 1.72 g (6.09 mmol, 72%) of the title compound as a colorless oil. MS (FAB): m/e 283 (M+1). ##STR36## Prep. 2E:

Dissolve the product (1.50 g, 5.06 mmol) of Prep. 2A in dry THF (20 mL) and cool to -78° C. under N₂. Add [(CH₃)₃ Si]₂ NLi (5.5 mL of 1.0 M in THF, 5.5 mmol) and stir at -78° C. for 1 h. Add bromomethylcyclopropane (0.820 g, 0.59 mL, 6.07 mmol), warm slowly to 23° C. and stir for 16 h. Add saturated aqueous NH₄ Cl (40 mL), extract with EtOAc (3×30 mL), wash combined organic extracts with saturated aqueous NaCl, dry (MgSO₄), filter and concentrate. Purify by chromatography (175 mL of flash silica gel; eluant: 2% CH₃ OH--CH₂ Cl₂ then 4% CH₃ OH--CH₂ Cl₂). Combine appropriate fractions and concentrate to give 0.93 g (2.65 mmol, 53%) of the title compound as a colorless oil. MS (FAB): m/e 351 (M+1) ##STR37## Prep. 2F:

Treat the product of Prep. 2A in a procedure similar to that described for Prep. 2E, using allyl bromide. MS (Cl/CH₄): m/e 337 (M+1).

Step 4: Separately dissolve the products of Prep. 2A to 2F in CH₂ Cl₂, add trifluoroacetic acid and stir at 23° C. for 4 h. Concentrate, add 1 N NaOH, extract with CH₂ Cl₂, dry the combined organic extracts (MgSO4), filter and concentrate to obtain the corresponding substituted piperidines:

    ______________________________________                                         Prep.                                                                               Substituted Piperidine  Data                                              ______________________________________                                           2-A                                                                                                         MS (Cl/CH                                                                    .sub.4):  m/e 197 (M + 1)                            - 2-B                                                                                                      MS (Cl/CH.sub.4):  m/e 183 (M + 1)                                             - 2-C                                                                          MS (Cl/CH.sub.4):  m/e 213 (M + 1)                                             - 2-D                                                                          MS (Cl/  isobutane):  m/e 183 (M + 1)                                          - 2-E                                                                          MS (Cl/CH.sub.4):  m/e 251 (M + 1)                                             - 2-F                                                                          MS (Cl/CH.sub.4):  m/e 237 (M + 1)              ______________________________________                                    

Preparation 3 ##STR44## Step 1: Using the procedures of Preparation 2, substitute 4-amino-1-benzylpiperidine for 4-aminomethyl-1-(1,1-dimethylethyloxycarbonyl)-piperidine in Prep. 2, Step 2A and proceed through Prep. 2, Step 3.

Step 2: Treat palladium hydroxide (2.0 g) in EtOAc (100 mL) with the product of Step 1 (25.0 g, 0.0918 mol) in EtOAc (200 mL) and (tBOC)₂ O in EtOAc (200 mL). Shake the resulting mixture on a Parr shaker at 50 psi of H₂ pressure for 3 h then add more palladium hydroxide catalyst (2 g) and shake for 16 h. Filter off catalyst and wash with EtOAc. Concentrate and purify by chromatography (silica gel; eluant: 5% CH₃ OH--CH₂ Cl₂). Combine appropriate fractions and concentrate to give 24.37 g of the product as a white solid. MS (FAB): m/e 283 (M+1).

Step 3: Treat the product of Step 2 according to a procedure similar to that described for Preparation 2F. MS (Cl/CH₄): m/e 267 (M-55).

Step 4A: Treat the product of Step 3 (5.17 g, 16.0 mmol) in EtOAc (90 mL) and H₂ O (90 mL) with NalO₄ (20.57 g, 96.2 mmol) and RuO₂ (0.064 g, 0.48 mmol). Stir at 23° C. for 5 h, add 1 N HCl (20 mL) and filter. Wash solid with EtOAc and H₂ O. Separate layers of filtrate and extract with EtOAc. Dry combined organic extracts (MgSO₄), charcoal, and concentrate to give 5.10 g of the title compound. MS (FAB): m/e 341 (M+1). ##STR45## Step 4B: Treat the product of Preparation 2F according to a procedure similar to that described in Step 4A to give the protected amino acid.

Step 5A: The substituted piperazines 3A5 to 3H5 as well as 3N5 and 3O5 are prepared in a similar procedure using the appropriate amine. Treat the product of Step 4A (1.00 g, 2.94 mmol) in CH₂ Cl₂ (20 mL) with carbonyl-diimidazole (0.57 g, 3.53 mmol) and stir at 23° C. for 4 h. Add the appropriate amine and stir for 16 h. Add 1 N HCl and extract with CH₂ Cl₂. Dry the combined organic extracts (MgSO₄), filter, and concentrate. Purify by chromatography (silica gel; eluant: CH₃ OH--CH₂ Cl₂). Combine appropriate fractions and concentrate to give 3A5 to 3H5 and 3N5-3O5.

Step 5B: The compounds 3I5 to 3K5 are prepared in a similar procedure to that described in Step 5A substituting the product of Step 4B for the product of Step 4A using the appropriate amine.

Preparation 3L5: Treat the product of Preparation 3, Step 2 according to a procedure similar to that described for Preparation 2F, substituting the product of Example 18L, Step 2, in place of allyl bromide to obtain the title compound.

    __________________________________________________________________________     Prep                                                                              Substituted Piperidine         MS                                           __________________________________________________________________________       3A5                                                                                                              (FAB) m/e 368  (M + 1)                        - 3B5                                                                                                           (Cl/CH.sub.4) m/e  355 (M + 1)                                                 - 3C5                                                                          (Cl/CH.sub.4) m/e  284 (M - 55)                                                - 3D5                                                                          (FAB) m/e 410  (M + 1)                        - 3E5                                                                                                           (FAB) m/e 394  (M + 1)                        - 3F5                                                                                                           (FAB) m/e 396  (M + 1)                        - 3G5                                                                                                           (FAB) m/e 384  (M + 1)                        - 3H5                                                                                                           (FAB) m/e 424  (M + 1)                        - 3I5                                                                                                           (FAB) m/e 398  (M + 1)                        - 3J5                                                                                                           (Cl/CH.sub.4) m/e  354 (M + 1)                                                 - 3K5                                                                          (Cl/CH.sub.4) m/e  424 (M + 1)                                                 - 3L5                                                                          (FAB) m/e 370  (M + 1)                        - 3N5                                                                                                           (FAB) m/e 423  (M + 1)                        - 3O5                                                                                                           (FAB) m/e 426  (M + 1)                     __________________________________________________________________________

Step 6: The compounds 3A6 to 3N6 are prepared by treating the products 3A5 to 3O5 (Step 51 according to a procedure similar to Prep. 2, Step 4. Preparation of 3O6 is carried out by treating the product of Preparation 3, Step 1 according to a procedure similar to that described for Preparation 3, Step 2, omitting the (t-BOC)₂ O to obtain the title compound.

    __________________________________________________________________________     Preparation                                                                          Substituted Piperidine       MS                                          __________________________________________________________________________       3A6                                                                                                               (Cl/CH##                                                                     .sub.4) m/e  268 (M + 1)                       - 3B6                                                                                                            (Cl/CH.sub.4) m/e  254 (M + 1)                                                 - 3C6                                                                          (FAB) m/e  240 (M + 1)                       - 3D6                                                                                                            (Cl/CH.sub.4) m/e  310 (M + 1)                                                 - 3E6                                                                          (Cl/CH.sub.4) m/e  294 (M + 1)                                                 - 3F6                                                                          (Cl/CH.sub.4) m/e  284 (M + 1)                                                 - 3G6                                                                          (Cl/CH.sub.4) m/e  296 (M + 1)                                                 - 3H6                                                                          (FAB) m/e  324 (M + 1)                       - 3I6                                                                                                            (Cl/CH.sub.4) m/e  298 (M + 1)                                                 - 3J6                                                                          (Cl/CH.sub.4) m/e  254 (M + 1)                                                 - 3K6                                                                          (Cl/CH.sub.4) m/e  324 (M + 1)                                                 - 3L6                                                                          #STR71##                                     - 3M6                                                                                                            (FAB) m/e  323 (M + 1)                       - 3N6                                                                                                            (FAB) m/e  326 (M + 1)                       - 3O6                                                                                                            (FAB) m/e  183 (M + 1)                    __________________________________________________________________________

Preparation 4 ##STR75## Preparation 4A Step 1: Treat N-benzyl-piperidone (8.00 g, 0.0423 mol) in CH₂ Cl₂ with (CH₃)₃ SiCN(4.82 g, 0.0486 mol) and Znl₂ (0.68 g, 0.0021 mol). Stir at 23° C. for 16 h and concentrate. Add CH₃ OH saturated with NH₃ (30 mL) and heat at 40° C. Concentrate the resulting mixture, add CH₂ Cl₂ (200 mL), dry (MgSO₄), filter and concentrate to give 11.06 g of the desired product as a yellow oil. MS (Cl/CH₄): m/e 189 (M-26).

Step 2: Treat the product of Step 1 according to a procedure similar to that of Preparation 2, Steps 2A and 3. MS (Cl/CH₄): m/e 298 (M+1).

Step 3: Treat the product of Step 2 (1.50 g, 5.04 mmol) in t-BuOH (25 mL) with KOH (0.99 g, 17.64 mmol) and reflux for 30 min. Cool to 23° C. and concentrate. Add saturated NaCl (40 mL), extract with CH₂ Cl₂ (3×40 mL), dry (MgSO₄), filter and concentrate. Purify by flash chromatography (silica gel; eluant: 10% CH₃ OH--CH₂ Cl₂). Combine appropriate fractions and concentrate to give 0.98 g of the desired product as a yellow solid. M.p.=184-186° C. MS (FAB): m/e 298 (M-17).

Step 4: Treat the product of Step 3 (0.97 g, 3.08 mmol) in CH₃ OH (25 mL) with palladium hydroxide (0.40 g). Shake on Parr shaker at 50 psi of H₂ pressure for 16 h. Filter, wash with CH₃ OH and concentrate to give 0.69 g of the title compound as a white solid. m.p.=180-185° C. MS (FAB): m/e 210 (M-15) ##STR76## Preparation 4B Step 1: Treat the product of Prep. 4A, Step 2 (1.50 g, 5.04 mmol) in CH₂ Cl₂ (25 mL) with trichloroethyl chloroformate (TROC-Cl) (1.39 g, 6.55 mmol). Stir at 23° C. for 16 h. Add 0.25 N NaOH (40 mL), extract with CH₂ Cl₂ (3×40 mL), dry (MgSO₄), filter and concentrate. Purify by flash chromatography (silica gel; eluant: 1:1 EtOAc: hexane to 2:1 EtOAc:hexane). Combine appropriate fractions and concentrate to give 1.31 g of the desired compound as a white solid. m.p.=185-186° C. MS (Cl/CH₄): m/e 382 (M+1).

Step 2: Treat the product of Step 1 (1.30 g, 3.40 mmol) in THF (20 mL) with HOAc (1.9 mL, 34.0 mmol) and zinc (2.22 g, 34.0 mmol). Stir at 23° C. for 18 h. Add H₂ O (10 mL), filter and wash with EtOAc. Add 6.25 N NaOH to filtrate, extract with CH₂ Cl₂, dry (MgSO₄), filter and concentrate to give 0.70 g of the title compound as a white solid. MS (Cl/CH₄): m/e 208 (M+1).

Preparation 4C

Step 1: Treat 4-cyano-4-phenylpiperidine in CH₃ OH with 50% KOH/H₂ O and heat in a sealed tube at 180° C. for 2 h. Cool to 23° C. and concentrate give the desired compound.

Step 2: Treat the product of Step 1 with di-t-butyl-dicarbonate according to a procedure similar to that in Preparation 2 Step 1 to give the protected amino acid.

Step 3: Couple the product of Step 2 with morpholine according to a procedure similar to Example 8 using DMF as a solvent.

Step 4: Deprotect the amine using a procedure similar to Prep. 2, Step 4, optionally substituting HCl for TFA. ##STR77## Preparation 4D Step 1 Cool acetaldehyde (4.6 g, 105 mmol) and dimethylacetone dicarboxylate (7.1 g, 35 mmol) to 0° C. and treat with benzylamine (5.2 g, 49 mmol), 12 N HCl, (4.1 mL), and H₂ O (3 mL). Stir at 23° C. for 16 h. Concentrate the reaction mixture, add acetone (20 mL), filter and concentrate. Add 6 N HCl (30 mL) and heat at 80° C. for 16 h. Cool the resulting solution to 23° C., basify to pH 10 with KOH pellets and extract with CH₂ Cl₂ (3×80 mL). Dry combined organic extracts (MgSO₄), filter and concentrate. Purify by flash chromatography (silica gel; eluant: 10% EtOAc-hexane). Combine appropriate fractions and concentrate to give 1.8 g of yellow oil. MS (FAB) m/e 218 (M+1).

Step 2: Treat the product of Step 1 (1.7 g, 8.3 mmol) in CH₃ OH (10 mL) with H₂ NOH•HCl (1.2 g, 16.8 mmol) and CH₃ CO₂ Na (2.05 g, 25 mmol). Reflux for 4 h then cool to 23° C. and concentrate. Add saturated NH₄ Cl and extract with CH₂ Cl₂. Dry combined organic extracts (Na₂ SO₄), filter and concentrate to give 1.6 g of brown oil. MS (FAB) m/e 233 (M+1).

Step 3: Treat the product of Step 2 (1.5 g, 6.46 mmol) in EtOH (20 mL) with Raney nickel (1 g, washed with EtOH). Shake on Parr shaker at 41 psi of H₂ pressure for 16 h. Filter the reaction mixture and concentrate. Purify by flash chromatography (silica gel; eluant: 7% CH₃ OH with NH₃ --CH₂ Cl₂). Combine appropriate fractions and concentrate to give 0.85 g of a clear oil. MS (FAB) m/e 217 (M+1).

Step 4: Proceed in a similar fashion as described for Preparation 3O6 substituting the product of Step 3 for 4-amino-N-benzylpiperidine. MS (FAB) m/e 211 (M+1).

Preparation 5 ##STR78## Step 1: Cool a solution of (R)-(+)-4-benzyl-2-oxazolidinone (100 g, 563 mmol) and 1,10-phenanthroline (10 mg) in dry THF (1.25 L) to -78° C. and add n-BuLi via addition funnel at a rate such that the internal temperature remains ≦-70° C. Add n-BuLi (350 mL of 1.6 M in hexane, 350 mmol, 1 eq) until the reaction turns brown from the phenanthroline complex (ca. 349.5 mL). After 15 min, add 3-carbomethoxypropionyl chloride (69.5 mL, 564 mmol, 1 eq) over 10 min via syringe. Stir the resulting solution for 30 min at -78° C. Allow the mixture to warm to 23° C. then pour into EtOAc (2.5 L)/sat. NH₄ Cl (1 L). Wash the organic layer with saturated NH₄ Cl (1 L), saturated NaHCO₃ (2.5 L) and saturated NaCl (2.5 L), then dry (MgSO₄) and concentrate to obtain a yellow solid. Recrystallize the solid from hot isopropanol (820 mL) to give 157.9 g (542 mmol, 98%) of pure product as a colorless crystalline solid, mp 90-92° C.

Step 2: Cool a solution of TiCl₄ (419 mL of 1 M in CH₂ Cl₂, 419 mmol) in dry CH₂ Cl₂ (1.35 L) to 0° C. and treat with Ti(Oi-Pr)₄ (41.4 mL, 140 mmol) via syringe. After 10 min at 0° C., add diisopropylethyl amine (102.4 mL, 587 mmol) via dry addition funnel. Stir the resulting solution for 15 min at 0° C. then add the product of Step 1 (163.2 g, 561 mmol) in one portion. Stir the solution for 1 h at 0° C. then add freshly distilled acrylonitrile (147 mL, 2.24 mol) via dry addition funnel. Allow the resulting mixture to stand at 4° C. for 18 h then pour the reaction mixture into 25% aq NH₄ Cl (4 L)/EtOAc (6 L). Wash the organic layer with 12.5% aq NH₄ Cl (2×4 L), saturated NaHCO₃ (4 L), and saturated NaCl (4 L) then dry (MgSO₄) and concentrate. Dissolve the crude product in EtOAc and filter through a pad of silica gel (500g). Concentrate the filtrate (6 L) and recrystallize in hot CH₃ OH (4 ml/g) to give 116.5 g (338.3 mmol, 60%) of the pure product as a colorless crystalline solid, mp. 103-105° C.

Step 3: Treat a solution of the product of Step 2 (25g, 72.6 mmol) in CHCl₃ (100 mL) and CH₃ OH (400 mL) with PtO₂ (1.25 g) and place on the Parr shaker @45 psi. Shake for 24 h, then filter the mixture through a pad of Celite. Concentrate the filtrate to give 28.3 g of crude amine•HCl.

Step 4: Treat a solution of the product of Step 3 (72.6 mmol) in 1,2-dichloroethane (500 mL) with HOAc (6 mL, 105 mmol, 1.4 eq) followed by N-Boc-4 piperidone (14.6 g, 73.5 mmol, 1.01 eq,) and NaB(OAc)₃ H (25.7 g, 122 mmol, 1.7 eq). Stir for 1.0 h, then pour the mixture into CH₂ Cl₂ (1.4 L). Wash with saturated aqueous NaHCO₃ (2×560 mL), dry (MgSO₄) and concentrate to give 39.1 g of product.

Step 5: Stir a solution of the product of Step 4 (72.6 mmol) in CH₃ CN (500 mL) for 72 h at 50° C. Cool and concentrate to give 39.3 g of lactam.

Step 6: Treat a solution of the product of Step 5 (39.3g) (containing up to 72.6 mmol of a mixture of N-benzyl and N-methyl-cyclohexyl oxazolidinones) in CH₃ OH (150 mL), with NaOH (148 mL of 1 N aqueous NaOH, 2.2 eq). Stir for 6 h at 23° C., then concentrate. Add H₂ O (50 mL) and wash with EtOAc (3×200 mL) to remove the oxazolidinone. Acidify to pH 2 with 40 mL of 15% aq. HCl (4.4 M) and extract with CH₂ Cl₂ (4×200 mL). Dry (MgSO₄) the combined extracts and concentrate to give the pure acid as a colorless foam (22.3 g, 65.5 mmol, 96% ee). Recrystallize from hot acetone (18 mL/g, reflux, filter, cool, remove ca. 300 mL solvent on rotovap, seed and sonicate, cool to 10° C., isolate by filtration with 50 mL cold acetone wash) to give 16.5 g (48.5 mmol) of the pure product as a colorless solid, 16.5 g, (48.5 mmol, 67% from the product of Step 2); m.p. 145-147° C., >99% ee by chiral HPLC: Daicel Chiracel OD column, 85:15 hexane/isopropanol with 0.1% TFA).

Step 7: Treat a solution of the product of Step 6 (1 0.0g, 0.029 mol) in CH₂ Cl₂ (100 mL) with HOBT (6.0 g, 0.044 mol), the appropriate amine in THF (or dioxane) (0.044 mol), and DCC (9.1 g, 0.044 mol). Stir at 23° C. for 4 h. Filter and wash with 0.5 N NaOH. Separate layers, extract with CH₂ Cl₂, dry (MgSO₄), filter and concentrate. Purify by flash chromatography (silica gel, eluant: EtOAc then 5% CH₃ OH--EtOAc). Combine appropriate fractions and concentrate to give the product.

Step 8: Treat a solution of the product of Step 7 in CH₂ Cl₂ (125 mL) with TFA (25 mL). Stir at 23° C. for 4 h and concentrate. Add H₂ O (25 mL) and basiify with 20 wt % NaOH. Extract with 20% EtOH in CH₂ Cl₂ (7×100 mL), dry (MgSO₄), filter, and concentrate to give the products 5A to 5C.

    __________________________________________________________________________     Preparation                                                                           Substituted Piperidine                                                                               MS                                                __________________________________________________________________________       5A                                                                                                          (Cl/CH##                                                                     .sub.4) m/e  268 (M + 1)                             - 5B                                                                                                       (Cl/CH.sub.4) m/e  254 (M + 1)                     - 5C                                                                                                       (FAB) m/e  240 (M - 55)                         __________________________________________________________________________      ##STR82##      Step 1: Use the procedures of Example 11, Steps 1, 2 and 3, using      3,5-dichlorobenzoyl chloride in place of 3,5-bistrifluorobenzoyl chloride,      to obtain the corresponding ketone product.

Step 2: Treat the product of Step 1 with H₂ NOH HCl using a procedure similar to that described in Example 1 to obtain the title compound. Separation of the Z/E oxime mixture was performed by SiO₂ chromatography, eluting with mixtures of EtOAc:CH₂ Cl₂ to obtain the pure Z isomer as a colorless solid.

Method A:

Step 3A: Dissolve the product of Step 2 (134 g) in CH₂ Cl₂ (1.5 L). Treat sequentially with HOBT (44.6 g), BOC-D-phenylglycine (86.3 g) and DEC (65.9 g). Stir the mixture at 23° C. for 18 h, heat at reflux temperature for 2 h, recool to 23° C., treat with saturated NaHCO₃ solution (500 mL), separate the organic portion, dry (MgSO₄), filter and concentrate. Recrystallize the crude material, once from Et₂ O and twice from ^(i) Pr₂ O to give 1,1-dimethylethyl-[[[1-[[(3,5-dichlorobenzoyl)methylamine]methyl]-2-(3,4-dichlorophenyl)-5-methyl-hexen-1-ylidene]amino]oxy]-2-oxo-1-phenylethyl]carbamate (51 g). MS(FAB): m/e 722; [α]_(D) ²³ =-96.9° (c 0.4 CH₂ Cl₂); m.p. 98-102° C. (dec).

Step 4A: Dissolve the product of Step 3A, (25.2 g) in a 0.5M solution of H₂ NNH₂ in CH₂ Cl₂ :CH₃ OH (2:1) (200 mL) and stir at 23° C. for 30 min. Dilute the reaction mixture with CH₂ Cl₂ (100 mL), wash with H₂ O (100 mL), dry (MgSO₄), filter and concentrate. Purify the product by filtration through a pad of silica gel eluting with CH₂ Cl₂ to give the title compound (15.6 g). MS(FAB): m/e 647.

Method B:

Step 3B: Dissolve the product of Step 2 (750 g) in CH₂ Cl₂ (4.5 L) at 0° C. Treat sequentially with Et₃ N(233 g), DMAP (2.8 g) and pivaloyl chloride (204 g). Stir the mixture at low temperature, adding additional CH₂ Cl₂ (4 L) to maintain homogeneity. After 20 min., add H₂ O (100 mL), stir for 10 min., wash with saturated NaHCO₃ solution (2 L), H₂ O (2 L), dry (Na₂ SO₄) and concentrate at 23° C. Purify the oily product by filtration through a pad of silica gel eluting with CH₂ Cl₂ to give 3,5-dichloro-N-[3-(3,4-dichlorophenyl)-2-[[(2,2-dimethyl-3-oxopropoxy]imino]-6-methyl-5-heptenyl]-N-methylbenzamide (846 g).

Step 4B: The product from Step 3B is resolved using a Chiralpak AD™ column, eluting with mixtures of hexane/^(i) PrOH.

Step 5B: Treat the desired enantiomer from Step 4B according to a procedure similar to Method A-Step 4A to afford the title compound.

Preparation 7 ##STR83## Step 1: Treat a solution of 3,4-dichlorophenylacetic acid (25 g) with N-tBOC-sarcosine methyl ester (24.3 g) (prepared from sarcosine methyl ester HCl and di-t-butyidicarbonate) according to a procedure similar to Example 11, Step 2, to give the desired product (36 g).

Step 2: Treat 2-bromoethanol (107 g) in CH₂ Cl₂ (2 L) at 0° C. with t-butyidimethylsilylchloride (143 g), NEt₃ (130 g) and DMAP (11 g), allow the reaction mixture to warm to 23° C. and stir for 18 h. Wash the mixture with H₂ O (250 mL), 20% HCl (250 mL), 20% NH₄ OH (250 mL), dry (MgSO₄) and concentrate to give 2-(t-butyidimethylsilyloxy)-ethylbromide (197 g).

Step 3: Treat the product of Step 1 (57 g) in DMF (500 mL) at -10° C. with NaH (8.6 g, 60% disp. in oil) and stir for 1 h. Add 2-(t-butyidimethylsilyloxy)ethylbromide (51.3 g) and Nal (6.4 g) and stir for 18 h. Add EtOAc (400 mL) and saturated NaCl solution (300 mL). Separate the organic portion, dry (MgSO₄), filter and concentrate. Purify the crude oil by silica gel chromatography eluting with EtOAc/hexane mixtures to give product (60.1 g).

Step 4: Treat the product from Step 3 (28 g) with O-allylhydroxylamine HCl (17 g) according to a procedure similar to Example 1, to give the title compound (24.5 g).

EXAMPLE 1 ##STR84##

Treat a solution of the product of Preparation 1 (270 mg, 0.417 mmol) in dry pyridine (5 mL) with O-methoxylamine HCl (52 mg, 0.626 mmol, 1.5 eq) and heat to 60° C. for 30 min. Allow the vessel to cool to 23° C. and remove the pyridine in vacuo. Take up the crude product in a minimal amount of CH₂ Cl₂ (2 mL) and apply to a silica gel column (2.5 cm×15 cm) packed with hexane:EtOAc:triethylamine (66:33:1). Elute with the same solvent system to obtain 190 mg (0.281 mmol, 67%) of the title compound as a colorless foam.

HRMS (FAB, M+H⁺): m/e calc'd for [C₃₂ H₃₃ N₂ O₃ Cl₂ F₆ ]⁺ : 677.1772, found 677.1785.

Example 1 A (Z isomer) is prepared from the product of Preparation 1 in a procedure similar to that described for Example 1, using hydroxyl amine HCl as the starting material: ##STR85##

HRMS (FAB, M+H⁺): calc'd: 663.1616, found 663.1625.

EXAMPLE 2 ##STR86##

Treat a solution of Example 1A (400 mg, 0.603 mmol) in dry DMF (12 mL) at 0° C. with 60% NaH in mineral oil (48 mg), stir for 40 min and treat with methyl bromoacetate (60 μL, 0.633 mmol, 1.05 eq). Stir for 30 min, pour into EtOAc (250 mL)/half saturated NaHCO₃ (200 mL) and extract. Wash the organic layer with water (2×100 mL), then brine (10 mL) and dry over Na₂ SO₄. Purify the crude mixture by silica gel chromatography (4×15 cm; hex/EtOAc 1:1 we 2% NEt₃) to give 361.8 mg (0.492 mmol, 82%) of the pure product as an oil. HRMS (FAB, M+H⁺): m/e calic'd for [C₃₄ H₃₄ Cl₂ F₆ N₂ O₅ ]⁺ : 735.1827, found 735.1839.

Using a similar procedure, treat the product of Example 1A with the appropriate alkyl halide to obtain the following compounds 2A-2C:

    __________________________________________________________________________       #STR87##                                                                                           HRMS calc'd                                                Ex. R.sup.1 Alkyl Halide (FAB, M + H.sup.+) HRMS Found                       __________________________________________________________________________     2A                                                                               --CH.sub.2 CN                                                                           Br-acetonitrile                                                                           702.1725                                                                              702.1720                                            2B --CH.sub.2 CH.sub.2 OH 2-Br-1-(t-Bu-dimethyl- 707.1878 707.1856                                           silyloxy)-ethane*                                2C --(CH.sub.2).sub.3 -phthalyl N-(3-Br-propyl)- 850.2249 850.2248                                           phthalimide                                    __________________________________________________________________________      *Followed by desilylation with 1M TBAF in THF (3 h, 23° C.).      

EXAMPLE 3 ##STR88##

Treat a solution of the product of Example 2 (57 mg, 0.078 mmol) in MeOH (3 mL) at 0° C. with gaseous ammonia for 5 min. After venting 2-3 times, seal the vessel with a polypropylene cap and stir until TLC shows the reaction is complete (20 h) to give (56 mg, 0.078 mmol, >99%) of the pure product as a colorless powder. HRMS (FAB, M+H⁺): m/e calc'd for [C₃₃ H₃₃ Cl₂ F₆ N₃ O₄ ]⁺ : 720.1831, found 720.1841.

EXAMPLE 4 ##STR89##

Treat a suspension of H₂ NOH•HCl (47 mg, 0.68 mmol, 5 eq) in ethanol with KOH in MeOH (680 L, 0.68 mmol, 5 eq), sonicate for 5 min and then add to a solution of Example 2A (95 mg, 0.135 mmol) in ethanol (5 mL). Heat for 2.5 h at 60° C., filter, concentrate in vacuo and purify by silica gel chromatography (2.5×14 cm; CH₂ Cl₂ /MeOH (NH₃) 95:5) to give 98.3 mg (0.134 mmol, 99%) of the product as a film. HRMS (FAB): 735.1956 (M+H⁺).

EXAMPLES 5, 5A AND 5B ##STR90##

Using the procedures described below, compounds of the structural formula above were prepared, wherein the definitions of R¹ are shown in the following table:

    ______________________________________                                                              HRMS calc'd                                                 Ex. R.sup.1 (FAB, M + H.sup.+) HRMS Found                                    ______________________________________                                         5     --CH.sub.2 CN  634.1198   634.1206                                         5A --CH.sub.2 CH.sub.2 OH 639.1351 639.1342                                     - 5B                                                                                                          667.1351 639.1342                            ______________________________________                                    

EXAMPLE 5

Step 1: Prepare the allyl oxime ether of the product of Example 6, Step 7, employing O-allylhydroxylamine HCl as the alkoxyl amine.

Step 2: Remove the silyl protective group in a procedure similar to that described in Example 6, Step 8.

Step 3: Alkylate the hydroxyl group with 3,5-dichlorobenzylbromide in a procedure similar to that in Example 6, Step 9.

Step 4: Treat a solution of the product of step 3 (285 mg, 0.426 mmol) in 80% aqueous EtOH with Pd(PPh₃)₄ (25 mg, 0.021 mmol, 0.05 eq) and triethylammoniumformate (2.13 mL of 1M solution in THF, 5 eq) and stir at reflux for 4 h. Cool, concentrate and purify by silica gel chromatography (2.5×16.5 cm; hex/EtOAc 1:1 w/2% NEt₃) to give 185 mg (0.3095 mmol, 73%) as a film.

Step 5: Treat the product of step 4 in a similar fashion to Example 2, using BrCH₂ CN as the alkyl halide.

Example 5A: Treat the product of Example 5, step 4, in a similar fashion to Example 2, using 2-bromo-1-(t butyldimethylsiloxy)ethane as the alkyl halide, followed by desilylation (3 h, 23° C.) with 1M TBAF in THF.

Example 5B: Treat the product of Example 5, Step 5, in a similar fashion to Example 4 to obtain the desired product.

EXAMPLE 6 ##STR92## Steps 1-6: Prepare 3-(3,4-dichlorophenyl)-1-[[dimethyl(1,1-dimethyl-ethyl)silyl]oxy]-5-(4-hydroxy-4-phenyl-1-piperidinyl)-2-pentanone as described in U.S. Pat. No. 5,696,267.

Step7: Treat a solution of the product of Step 6 (6.6 g, 12.3 mmol) and NaOAc (6.05 g, 73.8 mmol) in EtOH (110 mL) and H₂ O (27 mL) with NH₂ OCH₃ •HCl. Stir the resulting solution for 12-18 hours at room temperature. Concentrate under reduced pressure and partition the resulting residue between CH₂ Cl₂ (100 mL) and H₂ O (100 mL). Extract the aqueous layer with CH₂ Cl₂ (3×100 mL), dry the combined organic layers over MgSO₄, filter and concentrate under reduced pressure to yield the crude product as a pale oil. This product is carried on without purification to the next step. HRMS (FAB, M+H⁺): m/e cal'd for [C₂₉ H₄₃ N₂ O₃ SiCl₂ ]⁺ : 565.2420, found 565.2410.

Step 8: Treat a solution of the crude oxime from Step 7 (≦12.3 mmol) in THF (400 mL) with TBAF (15.4 mL, 15.4 mmol, 1M in THF) at 0° C. Stir the solution for 2 hours. Quench the reaction with water and extract the aqueous phase with EtOAc (3×100 mL). Dry the combined organic layers over MgSO₄, filter and concentrate under reduced pressure to give the crude product as a yellow oil. Purify by silica gel chromatography (column: 7.5 cm×20 cm; pack column in CH₂ Cl₂ and elute using a gradient of 100% CH₂ Cl₂ to 5% CH₃ OH(NH₃)/CH₂ Cl₂) to obtain 16 g (29.9 mmol, 75% from Step 6) of the desired compound as a white solid. HRMS (FAB, M+H⁺): m/e cal'd for [C₂₃ H₂₉ N₂ O₃ Cl₂ ]⁺ : 451.1555, found 451.1553.

Step 9: Treat a solution of the product of Step 8 (200 mg, 0.44 mmol) in DMF at 0° C. with NaH (12 mg, 0.48 mmol). Stir the resulting mixture for 30 mins at 0° C. Add 2,4-difluorobenzylbromide (60 μL, 0.465 mmol) in one portion and remove cooling bath. Stir the reaction for 12-18 hours at room temperature. Quench the reaction with H₂ O and extract with EtOAC (3×30 mL). Dry the combined organic layers over MgSO₄, filter and concentrate under reduced pressure to give the crude compound as a yellow oil. Purify by silica gel chromatography (column: 2.5 cm×15 cm; pack column in 50% EtOAc/Hexane and elute using a gradient of 50-100% EtOAc/Hexane) to obtain 128mg (0.22 mmol, 50% ) of the title compound as a pale oil. HRMS (FAB, M+H⁺): m/e cal'd for [C₃₀ H₃₃ N₂ O₃ Cl₂ F₂ ]⁺ : 577.1836, found 577.1832.

EXAMPLE 7 ##STR93## Step 1: Add the product of Example 6, Step 8 (1.8 g) and TFA (0.31 μL) to o iodoxybenzoic acid (2.24 g) in DMSO (20 mL). Stir the mixture for 2 h and add ice/H₂ O (50 mL), conc. NH₄ OH soln. (5 mL) and EtOAc (50 mL). Stir the mixture and filter to remove solids. Wash the solid residue with H₂ O (2×20 mL) and EtOAc (2×20 mL). Combine the filtrates, separate the organic layer and wash with H₂ O (2×25 mL), dry over MgSO₄, filter and evaporate to give 3-(3,4-dichlorophenyl)-5-(4-hydroxy-4-phenyl-1-piperidinyl)-2-(2-methoxyimino)pentanal (1.8 g) as a foamy solid.

Mass spectrum (FAB): 449.

Step 2: Treat the product of Step 1 (0.2 g) in CF₃ CH₂ OH (5 mL) with 3 Å molecular sieves (1.0 g) and 3,5-bistrifluoromethylbenzylamine (0.14 g). Stir the mixture for 90 min. and add NaBH₃ CN(0.12 g). After 18 h. filter the reaction mixture through a pad of Celite, rinse the Celite with MeOH (10 mL) and evaporate the combined filtrates. Partition the residue between CH₂ Cl₂ (15 mL) and 20% KOH (15 mL). Separate the organic layer and extract the aqueous layer with CH₂ Cl₂ (2×20 mL). Combine the organic extracts, dry over MgSO₄, filter and evaporate to give a solid. Purify the crude by silica gel chromatography eluting with NH₃ /MeOH/CH₂ Cl₂ mixtures to give the title compound (0.1 g). HRMS (FAB, M+H⁺): m/e calc'd for [C₃₂ H₃₄ N₃ O₆ Cl₂ F₆ ]⁺ : 676.1932, found 676,1940.

EXAMPLE 7A:

3-(3,4-Dichlorophenyl)-5-(4-hydroxy4-phenyl-1-piperidinyl)-l -[[(2-methoxyphenyl)methyl]amino]-2-pentanone O-methyloxime.

Using the product of Example 7, Step 1 as starting material, prepare the compound of Example 7A using 2-methoxybenzylamine in a procedure similar to that described in Example 7, Step 2. HRMS (FAB, M+H⁺): m/e calc'd for [C₃₁ H₃₇ N₃ O₃ Cl₂ ]⁺ : 570.2290, found 570.2291

EXAMPLE 8 ##STR94##

Treat the product of Example 7A (50 mg) in CH₂ Cl₂ (5 mL) with HOBT (12.4 mg) and AcOH (1 mL) and cool to 0° C. To the cold solution, add DEC (17.6 mg) and stir for a further 18 h. Wash the reaction mixture with 10% NH₄ OH soln. (3 mL). Reextract the aqueous layer with CH₂ Cl₂ (3×3 mL), combine the organic portions, dry over MgSO₄, filter and evaporate to give a solid. Purify the crude by by silica gel chromatography eluting with NH₃ /MeOH/CH₂ Cl₂ mixtures to give the title compound (0.042 g).

Analysis: Calc'd for C₃₃ H₃₉ N₃ O₄ Cl₂. 0.5H₂ O; C, 63.76, H, 6.49, N, 6.76. Found: C, 63.83, H, 6.85, N, 6.95.

EXAMPLE 9 ##STR95## Step 1-7: Prepare 1-[[3,5-bis(trifluoromethyl)phenyl]methoxy]-3-(3,4-dichlorophenyl)-5-hydroxy-2-pentanone O-methyloxime as described in U.S. Pat. No. 5,696,267.

Step 8:

Dissolve oxalyl chloride (2.01 g, 15.82 mmol) in dry CH₂ Cl₂ (30 mL) and cool to -78° C. under N₂, add DMSO (2.47 g, 31.64 mmol) in dry CH₂ Cl₂ (12 mL) dropwise and stir at -78° C. for 15 mins. Add the product of Step 7 (6.56 g, 12.66 mmol) in dry CH₂ Cl₂ (20 mL) dropwise and stir at -78° C. for 3 h. Add diisopropylethylamine (4.91 g, 37.97 mmol) and stir at -78° C. for 1 h. Warm slowly to 0° C. and stir at 0° C. for 30 mins. Add water (150 mL) and extract with CH₂ Cl₂. Wash combined organic extracts with saturated aqueous NaCl, dry (MgSO₄), filter, and concentrate to give 6.53 g (12.66 mmol, 100%) of a yellow oil. MS (FAB): m/e 516 (M+1).

Step 9:

Dissolve the product (1.05 g, 2.03 mmol) of Step 8 and 4-phenylamino-piperidine (1.08 g, 6.13 mmol) in CF₃ CH₂ OH (10 mL), add crushed 3A sieves (1 g) and NaBH₃ CN(0.26 g, 4.07 mmol), and stir at 23° C. for 4 h. Concentrate and add water (60 mL) and EtOAc (60 mL). Filter through Celite, separate layers of filtrate and extract aqueous solution with EtOAc. Dry combined organic extracts (MgSO₄), filter and concentrate. Purify by chromatography (200 mL of flash silica gel; eluant:3% CH₃ OH--CH₂ Cl₂). Combine appropriate fractions and concentrate to give 0.98 g (1.45 mmol, 66%) of the title compound as a yellow oil. MS (FAB): m/e 676 (M+1)

The following compound of formula 9A is prepared by reacting the product of Example 9, Step 8, with an appropriate amine according to the procedure of Example 9, Step 9: ##STR96## MS(FAB): m/e 657 (M+1)

EXAMPLE 10 ##STR97##

Dissolve the product (0.380 g, 0.578 mmol) of Example 9A in THF (3 mL) and CH₃ OH (1 mL). Add 1 N KOH (2.7 mL, 2.70 mmol) and reflux for 16 h. Cool to 23° C. and add 1 N HCl (5 mL) and water (20 mL). Extract with CH₂ Cl₂ (3×20 mL), wash combined organic extracts with saturated aqueous NaCl, dry (MgSO₄), filter and concentrate to give 0.312 g (0.496 mmol, 86%) of the title compound as a yellow foam. MS (FAB): m/e 629 (M+1)

EXAMPLE 11 ##STR98##

Treat a suspension of sarcosine methyl ester hydrochloride (6.02 g, 43 mmole) in CH₂ Cl₂ (250 ml) at 0° C. with 3,5-bistrifluoromethyl benzoyl chloride (7.7 ml, 42.5 mmole) and Et₃ N(12.5 ml, 89.7 mmole). Stir the mixture at 20° C. for 1 h. Add water (150 ml) to the mixture and separate the organic layer. Dry (MgSO₄) and concentrate the organic layer to give crude product. Purify by silica gel chromatography (eluant: EtOAc:hexane (6:4)) to obtain the product 12 g (81%). ##STR99##

Treat a solution of 3,4-dichlorophenyl acetic acid (4.15 g, 20 mmole) in anhydrous THF (50 ml) at -60° C. with [(CH₃)₃ Si]₂ NLi (46.2 ml, 46.2 mmole) and slowly warm the mixture to 0° C. for 4h. Transfer this solution to a solution of the product of Step 1 (5.46 g, 16 mmole) in anhydrous THF (8 ml) at -30° C. Warm the reaction to -10° C. over 1 h, stir at 0° C. for 1 h and at 20° C. for 4h. Add 50% of aqueous HOAc (15 ml) and extract with EtOAc. Separate the organic layer, dry (MgSO₄) and concentrate to give the crude product. Purifiy by silical gel chromatography (eluant: hexane/EtOAc, 6:4) to give 5.21 g (69%) of the product. HRMS (FAB, M+H⁺)=mre calc'd for [C₁₉ H₁₄ NO₂ Cl₂ F₆ ]⁺ =472.0306, found 472.0306 ##STR100##

Treat a solution of the product of Step 2 (0.96 g, 2 mmole) in THF (6 ml) at -78° C. with [(CH₃)₃ Si]₂ NLi (2.5 ml, 2.5 mmole) and stir at -78° C. for 25 h. Add a solution of 1-bromo-3-methyl-2-butene (0.42 g) in THF (1 ml) to the above anion solution at -78° C., slowly warm the solution to 0° C. and stir at 20° C. for 2 h. Add saturated NH₄ Cl solution (5 ml), extract with EtOAc twice wash the combined EtOAc extracts with brine, dry (MgSO₄) and concentrate to give a crude product. Purify by column chromatography (silica gel; eluant: EtOAc:hexane, 2:8) to obtain 1 g of product (87%). MS (FAB, M+H⁺) m/e 540. ##STR101##

Treat a solution of the product of Step 3 (0.22 g, 0.4 mmole) in pyridine (3 ml) at 70° C. with methoxylamine HCl (95 mg, 1.14 mmole), stir at 70° C. for 6.5 h and then cool to 20° C. Add water to the reaction mixture, extract the solution with EtOAc, dry (MgSO₄) and concentrate the EtOAc extracts to give the crude product. Purify by silica gel chromatography (eluant: hexane:Et₂ O, 1:1) to give 74 mg (32%) of Z-isomer and 130 mg (56%) of E-isomer oximes. MS (FAB, M+H⁺)=m/e 569. ##STR102##

Treat the product of Step 4 (0.387 of E-isomer, 0.68 mmole) in a solution of EtOAc saturated with O₃ at -78° C. for 5 min. Purge the solution with N₂, add (CH₃)₂ S and warm the solution from -78° C. to 20° C. over 1 h. Concentrate the solution to give the desired aldehyde which is used directly in the next reaction without further purification. MS (FAB.M +H⁺)=m/e 543. ##STR103##

The Z isomer is prepared using a procedure similar to that described in Step 5A employing the Z isomer product of Step 4.

Step 6: Treat the product of Step 5 with 4-hydroxy-4-phenylpiperidine in a procedure similar to that described in Example 9, Step 9, to obtain the title compound (Z isomer) in overall 77% yield. HRMS(FAB,M+H⁺)=m/e calc'd for [C₃₃ H₃₄ N₃ O₃ Cl₂ F₆ ]⁺ :704.1881, found 704.1875. ##STR104## Step 1: Using the procedures of Example 11, substitute 3,5-dichlorobenzoyl chloride in place of 3,5-bistrifluorobenzoyl chloride in Step 1 and proceed through Steps 2, 3, 4 and 5, to obtain the title compound. Alternatively, to prepare optically active material, treat the product of Preparation 6 according to a procedure outlined in Example 13, Step 1.

Step 2: The following compounds of formula 12A to 12S are prepared by reacting the product of Step 1 with an appropriate amine (described in Preparations 3 and 4) according to a procedure similar to Example 9, Step 9. Stereoisomers are separated by HPLC on a chiral column using mixtures of hexane and isopropanol with 0.25% Et₂ NH added on a Daicel AD and/or OD column.

    __________________________________________________________________________       #STR105##                                                                    Example                                                                             Z                          MS (FAB): m/e                                  __________________________________________________________________________       12A                                                                                                            758 (M ##                                                                    + 1)                                              - 12B                                                                                                         714 (M + 1)                                     - 12C                                                                                                         784 (M + 1)                                     - 12D                                                                                                         717 (M + 1)                                     - 12E                                                                                                         756 (M + 1)                                     - 12F                                                                                                         755 (M + 1)                                     - 12G                                                                                                         668 (M + 1)                                     - 12H                                                                                                         668 (M - 17)                                    - 12I                                                                                                         742 (M + 1)                                     - 12J                                                                                                         744 (M + 1)                                     - 12K                                                                                                         770 (M + 1)                                     - 12L                                                                                                         784 (M + 1)                                     - 12M                                                                                                         728 (M + 1)                                     - 12N                                                                                                         636 (M + 1)                                     - 12O                                                                                                         733 (M + 1)                                     - 12P                                                                                                         783 (M + 1)                                     - 12Q                                                                                                         786 (M + 1)                                     - 12R                                                                                                         669 (M + 1)                                  __________________________________________________________________________      ##STR124##      Step 1: Treat the product of Preparation 6 with CH.sub.3 l using the      procedure of Example 2, followed by a procedure similar to Example 11,      Step 5, to obtain the title compound.

Step 2: Prepare the following compounds by reacting the product of Step 1 with an appropriate amine (for 13A to 13C see Preparation 5A-5C, for 13D see Preparation 3O6, for 13F treat the product of Preparation 5, Step 5, using a procedure similar to that described in Preparation 2, Step 4 to give the appropriate amino ester) according to a procedure similar to Example 9, Step 9, substituting NaB(OAc)₃ H in place of NaBH₃ CN and 1,2-dichloroethane for trifluoroethanol. Prepare 13G by treatment of Example 13D with MCPBA in CH₂ Cl₂ at 0° C. for 3 h; 13H is similarly prepared by treatment of 13B. Example 13E is prepared from Example 13F using standard saponification conditions similar to those described in Preparation 5, Step 6.

    __________________________________________________________________________       #STR125##                                                                                              HRMS                                                     Found                                                                        Ex. Z (FAB, M + H.sup.+) Additional Data                                     __________________________________________________________________________       13A                                                                                                             726.2145 [α].sub.D.sup.26 =                                            -41.1°  (c = 0.6, CH.sub.2                                              Cl.sub.2)                                        - 13B                                                                                                          712.1979 [α].sub.D.sup.26 =                                            -42.1°  (c = 0.6, CH.sub.2                                              Cl.sub.2)                                        - 13C                                                                                                          698.1829 [α].sub.D.sup.26 =                                            -42.2°  (c = 0.6, CH.sub.2                                              Cl.sub.2)                                        - 13D                                                                                                          641.1622 [α].sub.D.sup.26 =                                            -52.7°  (c = 0.28, EtOH)                  - 13E                                                                                                          699.1666 --                                    - 13F                                                                                                          713.1831 --                                    - 13G                                                                                                          -- MS = 659  (M + 1)                           - 13H                                                                                                          -- MS = 730  (M + 1)                        __________________________________________________________________________

EXAMPLE

    ______________________________________                                           #STR134##                                                                                              MS (FAB):                                              Example R1 m/e                                                               ______________________________________                                         14A        CH.sub.2 CH═CH.sub.2                                                                      754 (M + 1)                                            14B H 714 (M + 1)                                                              14C CH.sub.2 CN 753 (M + 1)                                                     - 14D                                                                                                   786 (M + 1)                                           - 14E                                                                                                   800 (M + 1)                                        ______________________________________                                    

Example 14A: Treat the product of Preparation 7 according to a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner as described in Example 9, Steps 8-9, using the product of Preparation 5A or 3A6 in place of 4-phenylamino-piperidine. Proceed in a manner similar to that of Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 3,5 dichlorobenzoyl chloride.

Example 14B: Treat the product of Example 14A using a procedure similar to Example 5, Step 4, to obtain the oxime. Altematively, for the preparation of optically active material, treat the product of Preparation 6 according to a procedure outlined in Example 11, Step 5B, followed by the procedure of Example 13A, Step 2.

Example 14C: Treat the product of Example 14B with BrCH₂ CN according to a procedure similar to Example 2A to give the title compound.

Example 14D: Use a procedure similar to that described in Example 4 using the product of Example 14C to obtain the title compound. The stereoisomers are separated by HPLC on a chiral column using mixtures of hexane and isopropanol with 0.25% Et₂ NH added on a Daicel AD and/or OD column.

Example 14E: Treat the product of Example 14C according to a procedure similar to that of Example 18M to obtain the title compound.

EXAMPLE

    ______________________________________                                           #STR137##                                                                                                MS (FAB):                                            Example R1 m/e                                                               ______________________________________                                         15A      H                  700 (M + 1)                                          15B CH.sub.2 CN 739 (M + 1)                                                    15C CH.sub.2 CH.sub.2 OH 744 (M + 1)                                            - 15D                                                                                                     757 (M + 1)                                         - 15E                                                                                                     771 (M + 1)                                         - 15F                                                                                                     772 (M + 1)                                         - 15G                                                                                                     841 (M + 1)                                         - 15H CH.sub.2 CH.sub.2 CH.sub.2 SO.sub.3 H 822 (M + 1)                        - 15I                                                                                                     796 (M + 1)                                         - 15J                                                                                                     851 (M + 1)                                         - 15K CH.sub.2 F 732 (M + 1)                                                  15L CH.sub.2 CH.sub.3 728 (M + 1)                                              15M                                                                                                        786 (M + 1)                                      ______________________________________                                    

Example 15A

Step 1: Treat the product of Preparation 7 using a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner as described in Example 9, Steps 8-9, using the product of Preparation 5B or 3B6 in place of 4-phenylamino-piperidine. Proceed in a manner similar to the procedure outlined in Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 3,5 dichlorobenzoyl chloride.

Step 2: Treat the resultant product using a procedure similar to Example 5, Step 4, to obtain the title compound.

Alternatively, for the preparation of optically active material, treat the product of Preparation 6 according to a procedure outlined in Example 11, Step 5, followed by the procedure of Example 13B, Step 2.

Example 15B: Treat the product of Step 2 with BrCH₂ CN according to a procedure similar to Example 2A to give the title compound.

Example 15C: Alkylate and deprotect the product of Example 15A according to a procedure similar to Example 2B to give the title compound.

Example 15D:

Step 1: Alkylate the product in Example 15A with allylchloroacetate according to a procedure similar to Example 2 to give the resulting allyl ester.

Step 2: Treat a solution of the product of Step 1 according to a procedure similar to that of Example 3 to give the title compound.

Example 15E: Treat a solution of Example 15D, Step 1, with CH₃ NH₂ according to a procedure similar to that of Example 3 to give the title compound.

Example 15F: Use a procedure similar to that described in Example 4 using the product of Example 15B to obtain the title compound. The stereoisomers are separated by HPLC on a chiral column using mixtures of hexane and isopropanol with 0.25% diethylamine added on a Daicel AD and/or OD column.

Example 15G:

Step 1: Deprotect the product of Example 15D, Step 1, using a procedure similar to Example 5, Step 4.

Step 2: Treat a solution of the product of Step 1 (82 mg) in dry CH₂ Cl₂ (1 mL) with NEt₃ (41μL), followed by BOP-Cl (36.5 mg). Stir 15 min at 23° C., then add 2-amino-1,3,4-thiadiazole (14 mg). Stir for 2 h, dilute with EtOAc (75 mL) and wash with 10% citric acid followed by H₂ O, and then by saturated NaHCO₃. Dry the organic layers (Na₂ SO₄), filter, concentrate and purify by silica gel chromatography to give the title compound.

Example 15H: Alkylate the product of Example 15A using a procedure similar to that described in Example 2 using 1,3-propane sultone in place of methyl bromoacetate to obtain the title compound.

Example 15I: Couple the product of Example 15G, Step 1, with H₂ NCH₂ CN using a procedure similar to that in Example 8 to give the title compound.

Example 15J: Couple the product of Example 15G, Step 1, with H₂ NCH₂ SO₃ H using a procedure similar to that in Example 8 to give the title compound.

Example 15K: Alkylate the product of Example 15A using a procedure similar to that described in Example 2 using BrCH₂ F in place of methyl bromoacetate to obtain the title compound.

Example 15L: Treat the product of Example 15A with iodoethane according to a procedure similar to Example 2 to give the title compound.

Example 15M: Treat the product of Example 15B according to a procedure similar to that of Example 18M to obtain the title compound.

EXAMPLE

    ______________________________________                                           #STR145##                                                                    Example                                                                               R1                  Physical Data                                       ______________________________________                                         16A    H                   MS = 687 (M + 1)                                      16B CH.sub.2 CN --                                                             16C CH.sub.2 CH.sub.2 OH --                                                     - 16D                                                                                                    -- R146##                                            - 16E                                                                                                    -- R147##                                            - 16F                                                                                                    -- R148##                                            - 16G                                                                                                    -- R149##                                            - 16H CH.sub.2 CH.sub.2 CH.sub.2 SO.sub.3 H --                                 - 16I                                                                                                    -- R150##                                            - 16J                                                                                                    -- R151##                                            - 16K CH.sub.2 F --                                                           16L CH.sub.2 CH.sub.3 --                                                        - 16M                                                                                                    --TR152##                                         ______________________________________                                    

Example 16A:

Step 1: Treat the product of Preparation 7 using a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner as described in Example 9, Steps 8-9, using the product of Preparation 5C or 3C₆ in place of 4-phenylamino-piperidine. Proceed in a manner similar to the procedure outlined in Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 3,5 dichlorobenzoyl chloride.

Step 2: Treat the resultant product using a procedure similar to Example 5, Step 4, to obtain the title compound.

Alternatively, for the preparation of optically active material, treat the product of Preparation 6 according to a procedure outlined in Example 11, Step 5, followed by the procedure of Example 13C, Step 2.

Example 16B: Treat the product of Step 2 with the BrCH₂ CN according to a procedure similar to Example 2A to give the title compound.

Example 16C: Alkylate and deprotect the product of Example 16A according to a procedure similar to Example 2B to give the title compound.

Example 16D:

Step 1: Alkylate the product in Example 16A with allylchloroacetate according to a procedure similar to Example 2 to give the resulting allyl ester.

Step 2: Treat a solution of the product of Step 2 according to a procedure similar to that of Example 3 to give the title compound.

Example 16E Treat a solution of Example 16D, Step 1, with CH₃ NH₂ according to a procedure similar to that of Example 3 to give the title compound.

Example 16F: Use a procedure similar to that described in Example 4 using the product of Example 16B to obtain the title compound. The stereoisomers are separated by HPLC on a chiral column using mixtures of hexane and isopropanol with 0.25% Et₂ N added on a Daicel AD and/or OD column.

Example 16G:

Step 1: Deprotect the product of Example 16D, Step 1, using a procedure similar to Example 5, Step 4.

Step 2: Treat the product of Step 1 according to a procedure similar to Example 15G, Step 2, to give the title compound.

Example 16H: Alkylate the product of Example 16A using a procedure similar to that described in Example 2 using 1,3-propane sultone in place of methyl bromoacetate to obtain the title compound.

Example 16I: Couple the product of Example 16G, Step 1, with H₂ NCH₂ CN using a procedure similar to that in Example 8 to give the title compound.

Example 16J: Couple the product of Example 16G, Step 1, with H₂ NCH₂ SO₃ H using a procedure similar to that in Example 8 to give the title compound.

Example 16K: Alkylate the product of Example 16A using a procedure similar to that described in Example 2 using BrCH₂ F in place of methyl bromoacetate to obtain the title compound.

Example 16L: Treat the product of Example 16A, Step 2 with CH₃ CH₂ according to a procedure similar to Example 2 to give the title compound.

Example 16M: Treat the product of Example 16B according to a procedure similar to Example 18M to obtain the title compound.

EXAMPLE

    __________________________________________________________________________       #STR153##                                                                                            HRMS calc'd                                                                           HRMS                                              Example R1 (FAB, M + H.sup.+) Found                                          __________________________________________________________________________     17A  H                  627.1463                                                                              627.1457                                          17B CH.sub.3 641.1620 641.1622                                                 17C CH.sub.2 CH.sub.2 OH 671.1725 671.1733                                     17D CH.sub.2 CN 666.1572 666.1591                                               - 17E                                                                                                        699.1787 699.1778                                - 17F                                                                                                        684.1678 684.1639                                - 17G                                                                                                        712.1991 712.1979                                - 17H                                                                                                        698.1834 698.1829                                - 17I                                                                                                        768.1460 768.1466                                - 17J CH.sub.2 CH.sub.2 CH.sub.2 OH 685.1882 685.1882                         17K CH.sub.2 CH.sub.2 CH.sub.2 SO.sub.3.sup.- NH.sub.4.sup.+ 749.1501                                       749.1508                                           - 17L                                                                                                        700.1627 700.1618                                - 17M                                                                                                        741.2256 741.2263                                - 17N                                                                                                        747.1708 747.1716                                - 17O                                                                                                        733.1552 733.1553                                - 17P                                                                                                        731.1759 731.1754                                - 17Q                                                                                                        714.1784 714.1795                                - 17R                                                                                                        728.1940 728.1953                                - 17S CH.sub.2 F 659.1526 659.1520                                             - 17T                                                                                                        #STR166##                                     __________________________________________________________________________

Example 17A:

Step 1: Treat the product of Preparation 7 using a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner as described in Example 9, Steps 8-9, using the product of Preparation 3O6 in place of 4-phenylamino-piperidine. Proceed in a manner similar to the procedure outlined in Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 3,5 dichlorobenzoyl chloride.

Step 2: Treat the resultant product using a procedure similar to Example 5, Step 4, to obtain the title compound.

Alternatively, for the preparation of optically active material, treat the product of Preparation 6 according to a procedure outlined in Example 11, Step 5, followed by the procedure of Example 13D, Step 2.

Example 17B: Alkylate Example 17A with CH₃ 1 according to a procedure similar to Example 2 to give the title compound.

Example 17C: Alkylate and deprotect the product of Example 17A according to a procedure similar to Example 2B to give the title compound.

Example 17D: Alkylate Example 17A with BrCH₂ CN according to a procedure similar to Example 2A to give the title compound.

Example 17E: Use a procedure similar to that described in Example 4, using the product of 1 7D to obtain the title compound.

Example 17F:

Step 1: Alkylate Example 17A with allylchloroacetate according to a procedure similar to Example 2 to give the resulting allyl ester.

Step 2: Treat a solution of the product of Step 1 according to a procedure similar to that of Example 3 to give the title compound.

Example 17G: Treat a solution of Example 17F, Step 1, with (CH₃)₂ NH according to a procedure similar to that of Example 3 to give the title compound.

Example 17H: Treat a solution of Example 17F, Step 1, with CH₃ NH₂ according to a procedure similar to that of Example 3 to give the title compound.

Example 17I:

Step 1: Deprotect the product of Example 1 7F, Step 1, using a procedure similar to Example 5, Step 4.

Step 2: Treat the product of Step 1 according to a procedure similar to Example 15G, Step 2, to give the title compound.

Example 17J: Alkylate Example 17A with 3-bromo-1-t-butyidimethylsilyloxy propane and deprotect using a procedure similar to that described in Example 2B to obtain the title compound.

Example 17K: Alkylate Example 17A using a procedure similar to that described in Example 2 using 1,3-propane sultone in place of methyl bromoacetate to obtain the title compound.

Example 17L:

Step 1: Alkylate the product of Example 17A with methyl bromoacetate using a procedure similar to that described in Example 2 to obtain the methyl ester.

Step 2. Treat the product of Step 1 according to a procedure similar to that in Example 4 to give the title compound.

Example 17M:

Step 1: Alkylate the product of Example 17A with bromopropylphthalimide using a procedure similar to that described in Example 2C to obtain the protected propylamine.

Step 2. Treat the product of Step 1 with (CH₃)NH₂ according to a procedure similar to that in Example 3 to give the primary amine.

Step 3: Treat the product of Step 2 (150 mg) in CH₂ Cl₂ (3 mL) with methyl isocyanate (14.7 mg) and stir for 1 h. Evaporate the solvent and purify by silica gel chromatography using CH₂ Cl₂ /CH₃ OH saturated with ammonia to provide 137 mg (86%) of the title compound.

Example 17N:

Step 1: Cool a solution of Example 17J (1.0 g) in CH₂ Cl₂ (20 mL) and then treat with NEt₃ (507 μL) and mesyl chloride (170 μL). Warm the solution to 0° C. and stirr for 30 min. Pour into EtOAc/NaHCO₃. Wash the organic layer with H₂ O, brine and dry (Na₂ SO₄). Remove the solvent to provide the mesylate (quantitative).

Step 2: Treat a solution of the product of Step 1 in dry DMF with NaSCH₃. Stir the solution for 45 min, then pour the mixture into into EtOAc/aqueous NaHCO₃. Wash the organic layer with H₂ O and brine, dry (Na₂ SO₄) and purify by silica gel chromatography using EtOAc/NEt₃ to provide 282 mg (86%) of the methyl sulfide.

Step 3: Dissolve the product of Step 2 (64 mg) in THF (2 mL) and treat with t-butanol (500 μL), osmium tetroxide (56 μL of 2.5% solution in t-butanol), and NMO (31.6 mg) and stir the mixture for 2 h at 23° C. Pour the mixture into into EtOAc/aqueous NaHSO₄. Wash the organic layer with saturated aqueous NaHCO₃ and brine, dry (Na₂ SO₄), filter, concentrate and purify by silica gel chromatography using EtOAc/NEt₃ to provide 57 mg (85%) of the title compound.

Example 17O: Alkylate and deprotect the product of Example 17A according to a procedure similar to Example 17J substituting 2-bromo-1-t-butyidimethylsilyloxy ethane for 3-bromo-1-t-butyidimethylsilyloxy propane. Convert the product to the methyl sulfone according to a procedure similar to that described in Example 17N.

Example 17P: Dissolve the product of Example 17N Step 2 (128 mg) in CH₂ Cl₂ (5 mL) and treat with ReOCl₃ (PPh₃)₄ (7.5 mg) and phenyl sulfoxide (51 mg). Stir for 3 h at 23° C., then add ReOCl₃ (PPh₃)₄ (7.5 mg) and phenyl sulfoxide (51 mg) and stir at 23° C. for 15 h. Add H₂ O (20 mL) and extract with CH₂ Cl₂. Dry the organic layer with MgSO₄ and concentrate. Purify using silica gel chromatography (EtOAc/NEt₃ /CH₃ OH as eluant) to provide 95 mg (72%) of the title compound.

Example 17Q: Alkylate the product of Example 17A with the product of Example 18L, Step 2, using a procedure similar to that described in Example 18L, Step 3, to obtain the title compound.

Example 17R: Treat the product of Example 17L with excess diazomethane in Et₂ O to obtain the title compound.

Example 17S: Alkylate the product of Example 17A using a procedure similar to that described in Example 2 using BrCH₂ F in place of methyl bromoacetate to obtain the title compound.

Example 17T: Treat the product of Example 17D according to a procedure similar to that of Example 18M to obtain the title compound.

EXAMPLE

    ______________________________________                                           #STR167##                                                                                                 MS (FAB):                                           Example R.sup.1 m/e                                                          ______________________________________                                         18A     H                    587 (M + 1)                                         18B CH.sub.3 601 (M + 1)                                                       18C CH.sub.2 CH.sub.2 OH 631 (M + 1)                                           18D CH.sub.2 CN 626 (M + 1)                                                     - 18E                                                                                                      659 (M + 1)                                        - 18F                                                                                                      728 (M + 1)                                        - 18G CH.sub.2 CH.sub.2 CH.sub.2 OH 645 (M + 1)                                - 18H                                                                                                      714 (M + 1)                                        - 18I                                                                                                      728 (M + 1)                                        - 18J                                                                                                      730 (M + 1)                                        - 18K                                                                                                      727 (M + 1)                                        - 18L                                                                                                      674 (M + 1)                                        - 18M                                                                                                      673 (M + 1)                                        - 18N                                                                                                      688 (M + 1)                                     ______________________________________                                    

Example 18A:

Step 1: Treat the product of Preparation 7 using a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner as described in Example 9, Steps 8-9, using the product of Preparation 3O6 in place of 4-phenylamino-piperidine. Proceed in a manner similar to the procedure outlined in Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 3,5-dimethylbenzoyl chloride.

Step 2: Treat the resultant product using a procedure similar to Example 5, Step 4, to obtain the title compound.

Alternatively, for the preparation of optically active material, treat the product derived from a procedure similar to that of Preparation 6, Steps 1 and 2, substituting 3,5-dimethylbenzoyl chloride for 3,5-dichlorobenzoyl chloride in Step 1 and resolving the product of Step 2 in a procedure similar to that described in Preparation 6, Step 4B. Continue with the procedure outlined in Example 11, Step 5, followed by the procedure of Example 13D, Step 2.

Example 18B: Alkylate Example 18A with CH₃ according to a procedure similar to Example 2 to give the title compound.

Example 18C: Alkylate and deprotect the product of Example 18A using a procedure similar to Example 2B to give the title compound.

Example 18D: Alkylate Example 18A with BrCH₂ CN according to a procedure similar to Example 2A to give the title compound.

Example 18E: Use a procedure similar to that described in Example 4 using the product of 18D to obtain the title compound.

Example 18F:

Step 1: Alkylate Example 18A with allylchloroacetate according to a procedure similar to Example 2 to give the allyl ester.

Step 2: Deprotect the product of Step 1 using a procedure similar to Example 5, Step 4.

Step 3: Couple the product of Step 2 with 2-amino-1,3,4-thiadiazole using a procedure similar to Example 15G, Step 2 to give the title compound.

Example 18G: Alkylate Example 18A with 3-bromo-1-t-butyidimethylsilyloxy propane and deprotect using a procedure similar to that described in Example 2B to obtain the title compound.

Example 18H: Treat the product of Example 18F, Step 1, with morpholine at 65° C. using a procedure similar to that in Example 3 to give the title compound.

Example 18I: Treat the product of Example 18F, Step 1, at 23° C. with n-methylpiperazine using a procedure similar to that in Example 3 to give the title compound.

Example 18J: Couple the product of Example 18F, Step 2, with thiomorpholine using a procedure similar to that in Example 8 to give the title compound.

Example 18K: Couple the product of Example 18F, Step 2, with 2-aminothiazole using a procedure similar to that in Example 18F, Step 3, to give the title compound.

Example 18L:

Step 1: Treat a solution of CH₃ ONH₂ •HCl (2.5g) in H₂ O (40 mL) with NaHCO₃ (5g). Cool to 0° C. and add a solution of ClCH₂ COCl (2.4 mL) in THF (20 mL) at a rate to maintain the internal temperature at 0-3° C. Upon complete addition, warm to 23° C. and stir for 2 h. Adjust pH to 5 (Na₂ CO₃), remove THF in vacuo, add NaCl and extract with CH₂ Cl₂. Dry the organic layer (Na₂ SO₄), filter and concentrate to give the α-chloroamide.

Step 2: Treat a solution of the product of Step 1 (131 mg) in acetone (1 mL) with Nal (158 mg). Stir for 7 h at 23° C., remove the solvent in vacuo, redissolve in THF, filter through Celite and concentrate to give the iodo-amide.

Step 3: Alkylate Example 18A with the product of Example 18L, Step 2, using a procedure similar to that described in Example 2 using 2.5 eq. of NaH to obtain the title compound.

Example 18M: Use a procedure similar to that described in Example 4 using the product of 18D and the following modifications: substitute CH₃ ONH₂ •HCl for HONH₂ •HCl, use 2,2,2-trifluoroethanol as the solvent, and stir at 70° C. for 1 week to obtain the title compound.

Example 18N: Treat the product of 18C according to a procedure similar to Example 17M, Step 3.

EXAMPLE

    ______________________________________                                           #STR177##                                                                                                 MS (FAB):                                           Example R1 m/e                                                               ______________________________________                                         19A       H                  672 (M + 1)                                         19B CH.sub.2 CN 711 (M + 1)                                                     - 19C                                                                                                      744 (M + 1)                                        - 19D                                                                                                      813 (M + 1)                                        - 19E                                                                                                      759 (M + 1)                                     ______________________________________                                    

Example 19A:

Step 1: Treat the product of Preparation 7 using a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner to Example 9, Steps 8-9, using the product of Preparation 5A or 3A6 in place of 4-phenylamino-piperidine. Proceed in a manner similar to the procedure outlined in Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 3,5-dimethylbenzoyl chloride.

Step 2: Treat the product of Step 1 using a procedure similar to Example 5, Step 4, to obtain the title compound.

Alternatively, for the preparation of optically active material, treat the product derived from a procedure similar to that of Preparation 6, Steps 1 and 2, substituting 3,5-dimethylbenzoyl chloride for 3,5-dichlorobenzoyl chloride in Step 1 and resolving the product of Step 2 in a procedure similar to that described in Preparation 6, Step 4B. Continue with the procedure outlined in Example 11, Step 5, followed by the procedure of Example 13A, Step 2.

Example 19B: Alkylate the product in Example 19A with BrCH₂ CN using a procedure similar to Example 2A to give the title compound.

Example 19C: Use a procedure similar to that described in Example 4 using the product of Example 19B to obtain the title compound.

Example 19D:

Step 1: Alkylate the product in Example 19A with allylchloroacetate according to a procedure similar to Example 2 to give the allyl ester.

Step 2: Treat the product of Step 1 using a procedure similar to Example 5, Step 4.

Step 3: Couple the product of Step 2 with 2-amino-1,3,4-thiadiazole according to a procedure similar to Example 15G, Step 2, to give the title compound.

Example 19E: Alkylate the product of Example 19A with the product of

Example 18L, Step 2, using a procedure similar to that described in Example 2 using 2.5 eq. of NaH to obtain the title commpound.

EXAMPLE

    ______________________________________                                           #STR181##                                                                                              MS (FAB):                                              Example R.sup.1 m/e                                                          ______________________________________                                           20A                                                                                                      686 (M ##                                                                    + 1)                                                    - 20B CH.sub.2 CH.sub.2 OH 658 (M + 1)                                      ______________________________________                                    

Example 20A:

Step 1: Treat the product of Preparation 7 using a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner to Example 9, Steps 8-9, using 4-hydroxy-4-phenylpiperidine in place of 4-phenylamino-piperidine. Proceed in a manner similar to the procedure of Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 3,5-dimethyoxybenzoyl chloride.

Step 2: Treat the product of Step 1 using a procedure similar to Example 5, Step 4.

Step 3: Alkylate the product of Step 2 with BrCH₂ CN according to a procedure similar to Example 2A to give the title compound.

Step 4: Use a procedure similar to that described in Example 4 using the product of Step 3 to obtain the title compound.

Example 20B: Alkylate the product of Example 20A, Step 2 with 2-bromo-1-t-butyldimethylsilyloxy ethane and deprotect using a procedure similar to that described in Example 2B to obtain the title compound.

EXAMPLE

    ______________________________________                                           #STR183##                                                                                              MS (FAB):                                              Example R.sup.1 m/e                                                          ______________________________________                                         21A         CH.sub.2 CN   672 (M + 1)                                             - 21B                                                                                                   705 (M + 1)                                        ______________________________________                                    

Example 21A:

Step 1: Treat the product of Preparation 7 using a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner as described in Example 9, Steps 8-9, using the product of Preparation 3O6 in place of 4-phenylamino-piperidine. Proceed in a manner similar to the procedure outlined in Preparation 2, Step 4, optionally substituting HCl for TFA. Acylate the amine according to a procedure similar to Preparation 2, Step 2A, using 4-methyl-3,5-dimethoxybenzoyl chloride.

Step 2: Treat the product of Step 1 using a procedure similar to Example 5, Step 4.

Step 3: Alkylate the product of Step 2 with BrCH₂ CN using a procedure similar to Example 2A to give the title compound.

Example 21B: Treat the product of Example 21A using a procedure similar to that described in Example 4 to obtain the title compound.

EXAMPLE 22 ##STR185##

Use a procedure similar to that described in Example 20A using the product of Preparation 3O6 in place of 4-hydroxy-4-phenylpiperidine in Step 1 to obtain the title compound. HRMS (FAB, M+H⁺): m/e calc'd: 691.2778, found 691.2769.

EXAMPLE 23 ##STR186##

Use a procedure similar to that described in Example 20A using 3,4,5-trimethoxybenzoyl chloride as the acid chloride in Step 1 to obtain the title compound; MS (FAB): m/e 716 (M+1).

EXAMPLE 24 ##STR187##

Use a procedure similar to that described in Example 11, using the product of Preparation 3O6 as the amine in Step 6 to obtain the title compound. HRMS (FAB, M+H⁺): m/e calc'd: 709.2147 found 709.2138.

EXAMPLE 25 ##STR188##

Using a procedure similar to that of Example 2, methylate product of Example 3H5, replacing methyliodide with methylbromoacetate. Deprotect the resulting product and couple to the product of Example 12, Step 1, using the proccedure of Example 9, Step 9, to obtain the title compound. HRMS (FAB, M+H⁺): m/e calc'd: 798.2537; found 798.2538.

EXAMPLE 26 ##STR189##

Use a procedure similar to that described in Example 17B using 3-chloro-5-methyl-benzoyl chloride instead of 3,5-dichlorobenzoyl chloride (Step 1, Example 17A) to obtain the title compound. HRMS (FAB, M+H⁺): m/e calc'd: 621.2166, found 621.2178

EXAMPLE 27 ##STR190##

Use a procedure similar to that described in Example 13A using 3-chloro-5-methyl-benzoyl chloride instead of 3,5-dichlorobenzoyl chloride to obtain the title compound. HRMS (FAB, M+H⁺): m/e calc'd: 706.2694, found 706.2701

EXAMPLE 28 ##STR191## Step 1: Condense glycine with 3,5-dichlorobenzoic acid according to a procedure similar to Example 8. Treat the resulting amide with NaH followed by iodoethane according to Example 2. Treat the resulting material with diazomethane to give N-ethyl-N-(3,5-dichlorobenzoyl)glycine methyl ester.

Step 2: Using the procedures of Example 11, Steps, 2-5, substituting the product of Step 1 for the product of Example 11, Step 1. Optically active material may be prepared using procedures similar to Preparation 6.

The following compounds are prepared by reacting the product of

Step 2 with an appropriate amine (for 28A to 28C, see Preparation 5A-5C; for 28D, see Preparation 3O6) according to a procedure similar to Example 9, Step 9, substituting NaB(OAc)₃ H for NaBH₃ CN and optionally substituting 1,2-dichloroethane for 2,2,2-trifluoroethanol.

    __________________________________________________________________________       #STR192##                                                                    Example                                                                             Z                       Data                                              __________________________________________________________________________       28A                                                                                                         #STR193##                                                                    --                                                   - 28B                                                                                                      MS (FAB):  m/e 728 (M + 1)                         - 28C                                                                                                      HRMS:  calc'd: 712.1991  found: 712.1986                                       - 28D                                                                          --TR196##                                       __________________________________________________________________________

EXAMPLE 29 ##STR197## Step 1: Add carbonyldlimidazole (24 g) to a solution of allyl-3,4-dichlorophenyl acetic acid (30.6 g) in anhydrous THF (600 mL). Stir the reaction mixture at 23° C. for 1 hr. In a separate flask, under N₂, dissolve potassium t-butoxide (16.8 g) in anhydrous THF (425 mL). Cool the solution to 0° C. and add CH₃ NO₂ (200 mL) over 30 min. Add the acyl imidazole solution to the potassium nitronate solution via addition funnel while keeping the internal temperature between 0° C. and 5° C. Remove the cooling bath and stir at 23° C. for 2 days. Cool the reaction mixture to 0° C. and pour into cold 1 M HCl (500 mL). Separate the organic layer, dry (MgSO₄) and concentrate to give 34 g of orange oil. MS (Cl+/CH₄) m/e 288 (M+1).

Step 2: Cool a solution of the product of Step 1 (12.2 g) in THF (34 mL) and HOAc (34 mL) to 0° C. and add H₂ O (17 mL). Add powdered Zn (15.6 g) in portions over 15 min. Stir the reaction mixture 15 mmn at 0° C. then warm to 23° C. Heat the reaction mixture to 40° C. for 3 min. Remove the heat and pour into H₂ O (150 mL) and THF (100 mL). Filter the mixture and wash the solids with THF and H₂ O. Concentrate the filtrate and wash the resulting orange material with Et₂ O and CH₂ Cl₂. Concentrate the filtrate to give 15.5 g orange oil. MS (Cl +/CH₄) m/e 259 (M+1).

Step 3: Cool a solution of the product of Step 2 (15.5 g) in THF (55 mL) and H₂ O (10 mL) to 0° C. and add 3,5-dichlorobenzoyl chloride (8.2 g), Hunig's base (12 mL) and DMAP (0.25 g). Warm the reaction mixture to 23° C. and stir for 16 h. Add EtOAc (300 mL) and wash with H₂ O (30 mL ), 1 M HCl (3×30 mL ), H₂ O (2×30 mL) and brine (2×40 mL), dry (MgSO₄), and concentrate. Purify (SiO₂ ; elute with gradient of 4% EtOAc/hexanes to 25% EtOAc/hexanes) to give 8.8 g of a solid. MS (Cl+/CH₄) m/e 432 (M+1).

Step 4: Treat a solution of the product of Step 3 (5.7 g, 13 mmol) in EtOH (100 mL) and H₂ O (25 mL) with CH₃ ONH₂ •HCl (5.54 g, 66.3 mmol) and stir at 23° C. for 2 days. Concentrate the mixture and add EtOAc (200 mL) and H₂ O (20 mL). Separate the layers and wash the organic layer with 1 M NaHCO₃ (2×20 mL) and brine (20 mL), dry (MgSO₄) and concentrate to an oil. Purify (SiO₂ ; elute with a gradient of 4% EtOAc/hexanes to 15% EtOAc/hexanes to give 1.8 g of clear oil. MS (Cl+/CH4) m/e 461 (M+1)

Step 5: Treat the product of Step 4 according to a procedure similar to Example 11, Step 5, to obtain the title compound. MS (FAB): m/e 464 (M+1).

The following compounds of formula 29A to 29D are prepared by reacting the product of Step 5 with an appropriate amine (for 29A to 29C, see Preparation 5A-5C; for 29D, see Preparation 3O6) according to a procedure similar to Example 9, Step 9.

    __________________________________________________________________________       #STR198##                                                                    Example                                                                             Z                       MS (FAB): m/e                                     __________________________________________________________________________       29A                                                                                                                #STR199##                                                                    --  No Sch #                                  - 29B                                                                                                             700 (M + 1)                                 - 29C                                                                                                             686 (M + 1)                                 - 29D                                                                                                             629 (M + 1)                              __________________________________________________________________________

EXAMPLE 30 ##STR203##

Treat the crude product from Example 7, Step 1, (1.65 g) with CH₃ NH₂ according to a procedure similar to Example 7, Step 2, to give the desired product (0. 6 g). MS(FAB): m/e 464.

Treat the product from Step 1 with the appropriate carboxylic acid according to a procedure similar to Example 8 to give the following compounds:

    __________________________________________________________________________       #STR204##                                                                                                       MS (FAB):                                     Example Carboxylic Acid XT m/e                                               __________________________________________________________________________       30A                                                                                                               #STR205##                                                                      617 (M ##                                                                    + 1)                                           - 30B                                                                                                            #STR207##                                                                      597 (M + 1)                                  - 30C                                                                                                            #STR209##                                                                      637 (M + 1)                                  - 30D                                                                                                            #STR211##                                                                      626 (M + 1)                                  - 30E                                                                                                            #STR213##                                                                      633 (M + 1)                                  - 30F                                                                                                            #STR215##                                                                      658 (M + 1)                                  - 30G                                                                                                            #STR217##                                                                      628 (M + 1)                                  - 30H                                                                                                            #STR219##                                                                      596 (M + 1)                                  - 30I                                                                                                            #STR221##                                                                      600 (M + 1)                               __________________________________________________________________________

EXAMPLE 31 ##STR223## Step 1: Treat the product of Preparation 7 according to a procedure similar to that described in Example 6, Step 8. Proceed in a similar manner as described in Example 9, Steps 8-9, using 4-phenyl-4-hydroxypiperidine in place of 4-phenylamino-piperidine.

Step 2: Treat the product of Step 1 using a procedure similar to Example 5, Step 4.

Step 3: Treat the product of Step 2 in a similar procedure to that of Preparation 2, Step 4.

Step 4: Treat the product of Step 3 (8.4 g) with 2-chloro-6-methylpyridine-4-carboxylic acid (3.84 g), according to a procedure similar to Example 8 to give product (5.9 g).

Step 5: Treat the product of Step 4 (3.65 g) with BrCH₂ CN(0.725 g) according to a procedure similar to Example 2A to give the product (1.9 g). Step 6: Treat the product of Step 5, (1.8 g) using a procedure similar to Example 4, to give the title compound (1.31 g). MS(FAB): m/e 675 (M+1).

EXAMPLE 32 ##STR224##

Alkylate and deprotect the product of Example 31, Step 4, using a procedure similar to Example 2B, to give the product. MS(FAB): m/e 647 (M+1).

EXAMPLE 33 ##STR225##

The title compound is prepared in a similar manner to the procedures of Example 32 by substituting 2-chloro-6-methylpyridine-4-carboxylic acid with 2-(dimethylamino)-6-methylpynidine4-carboxylic acid and the product of Preparation 3O6 for 4-hydroxy4-phenyl piperidine. MS(FAB): m/e 661 (M+1).

EXAMPLE 34 ##STR226##

Treat the crude product from Example 7, Step 1, according to a procedure similar to Example 30, substituting NH₄ OAc for MeNH₂ in Step 1 and using 3,5-bis(trifluoromethyl)benzoic acid in Step 2 to give the title compound. MS(FAB): m/e 690.

EXAMPLE 35 ##STR227##

Use a procedure similar to that described in Example 18B using the product of Preparation 5B in place of the product of Preparation 3O6 to obtain the title compound. HRMS (FAB): m/e 672 (M+1).

EXAMPLE 36 ##STR228## Step 1: 4-Piperidone hydrochloride (50 g) in saturated NaHCO₃ solution (1 L) was treated with a dioxane (30 mL) solution of benzyloxycarbonyl-chloride (53.4 mL). The solution was stirred for 18 h at room temperature then extracted with EtOAc (2×500 mL). The organic fractions were combined, washed with 1 N HCl (500 mL) and brine (500 mL), dried over MgSO₄, filtered and evaporated to afford 1-benzyloxycarbonyl-4-piperidone (75 g).

Step 2: 2-Bromothioanisole (13.7 g) in TMF (120 mL) was cooled to -78° C. (internal temperature) and treated with n-BuLi (36.5 mL, 2.5 M hexane solution). After 10-15 min., a THF (120 mL) solution of the product of Step 1 (12.5 g), also at -78° C., was added via a cannula. The combined mixture was stirred for a further 18 h, during which time it warmed to room temperature. The solution was treated with saturated NH₄ Cl solution (250 mL), EtOAc (250 mL) and the organic layer separated. The aqueous portion was extracted with additional EtOAc (2×250 mL). The organic extracts were combined, washed with H₂ O (250 mL) and brine (250 mL), dried over MgSO₄, filtered and evaporated to give an oil. Silica gel chromatography, eluting with EtOAc/hexane mixtures, gave 1-benzyloxycarbonyl-4-hydroxy-4-(2-methylthiophenyl)piperidine (9.61 g).

Step 3: The product of Step 2 (5.1 g) in CH₂ Cl₂ (50 mL) was treated sequentially with trifluoroacetic acid (8.9 mL) and triethylsilane (34.5 mL). After 18 h, the solution was treated with saturated NaHCO₃ (150 mL). After a further 1 h, the organic layer was separated and the aqueous extracted with CH₂ Cl₂ (100 mL). The organic extracts were combined, dried over Na₂ SO₄, filtered and evaporated. The crude product was purified by silica gel chromatography, eluting with EtOAc/hexane mixtures to give 1-benzyloxycarbonyl-4(2-methylthiophenyl)piperidine (3.3 g).

Step 4: The product of Step 3 (2.55 g) in CH₂ Cl₂ (30 mL) was cooled to 0° C. and treated with mCPBA (60%, 2.16 g) and stirred for 1 h. The reaction mixture was treated with ice (20 g), saturated NH₄ OH solution (20 mL) and stirred for 10 minutes. The organic layer was separated and the aqueous layer re-extracted with CH₂ Cl₂ (2×30 mL). The combined organic portions were dried over MgSO₄, filtered and evaporated to give a gum. Silica gel chromatography, eluting with EtOAc/CH₂ Cl₂ mixtures, gave 1-benzyloxycarbonyl-4-(2-methylsulfinylphenyl)piperidine (0.5 g).

Step 5: The product of Step 4 (0.5 g) was treated with trifluoroacetic acid (10 mL) and heated at reflux temperature for 45 minutes. The reaction mixture was cooled, diluted with toluene (40 mL) and evaporated. This procedure was repeated two more times. The residue was treated with CH₂ Cl₂ (70 mL) and adjusted to alkaline pH via addition of NH₄ OH solution. The organic layer was separated, dried over MgSO₄, filtered and evaporated to give 4-(2-methylsulfinylphenyl)piperidine (0.3 g).

Step 6: Using a procedure similar to Example 9, Step 9, the product of Step 5 is coupled to the appropriate O-methyloxime to obtain the title compound. Mass Spec (FAB) 684 (100%).

Using the procedures of Steps A through E of Example 36, starting with 3-bromothioanisole and 4-bromothioanisole, the corresponding 4-(3-methylsulfinylphenyl) piperidine and 4-(4-methylsulfinylphenyl)piperidines were obtained.

EXAMPLE 37 ##STR229## Step 1: Treat the product of Preparation 7 as described in Example 20A, acylating the amine using 3,4,5-trimethoxybenzoyl chloride.

Step 2: Treat the product of Step 1 using a procedure similar to Example 5, Step 4.

Step 3: Alkylate the product of Step 2 with BrCH₂ CN according to a procedure similar to Example 2A.

Step 4: Treat a solution of the product of Step 3 (2.5 g) in CH₃ OH (37 mL) with NaOCH₃ (200 mg). Stir for 18 h. Treat the resulting solution with CH₃ ONH₂ •HCl and stir for 3 h. Remove the solvent and re-suspend in CH₂ Cl₂, dry over MgSO₄, filter, concentrate in vacuo and purify by silica gel chromatography (5×20 cm; 5% CH₃ OH/CH₂ Cl₂) to give 2.2 g of the desired product as a white foam. HRMS (FAB) 730.2774 (M+H⁺).

The following formulations exemplify some of the dosage of this invention. In each, the term "active compound" refers to a ound of formula 1.

EXAMPLE A

    ______________________________________                                         Tablets                                                                          No.    Ingredient          mg/tablet                                                                              mg/tablet                                 ______________________________________                                         1    Active Compound     100       500                                           2 Lactose USP 122 113                                                          3 Corn Starch, Food Grade, as a 10% 30 40                                       paste in Purified Water                                                       4 Corn Starch, Food Grade 45 40                                                5 Magnesium Stearate 3 7                                                        Total 300 700                                                               ______________________________________                                    

Method of Manufacture

Mix Item Nos. 1 and 2 in suitable mixer for 10-15 minutes. Granulate the mixture with Item No. 3. Mill the damp granules through a coarse screen (e.g., 1/4", 0.63 cm) if necessary. Dry the damp granules. Screen the dried granules if necessary and mix with Item No. 4 and mix for 10-15 minutes. Add Item No. 5 and mix for 1-3 minutes. Compress the mixture to appropriate size and weight on a suitable tablet machine.

EXAMPLE B

    ______________________________________                                         Capsules                                                                         No.       Ingredient      mg/tablet                                                                             mg/tablet                                   ______________________________________                                         1       Active Compound 100      500                                             2 Lactose USP 106 123                                                          3 Corn Starch, Food Grade 40 70                                                4 Magnesium Stearate NF 4 7                                                     Total 250 700                                                               ______________________________________                                    

Method of Manufacture

Mix Item Nos. 1, 2 and 3 in a suitable blender for 10-15 minutes. Add Item No. 4 and mix for 1-3 minutes. Fill the mixture into suitable two-piece hard gelatin capsules on a suitable encapsulating machine.

EXAMPLE C

    ______________________________________                                         Sterile Powder for Injection                                                        Ingredient        mg/vial mg/vial                                         ______________________________________                                         Active sterile powder                                                                             100     500                                                 ______________________________________                                    

For reconstitution add sterile water for injection or bacteriostatic water for injection.

The in vitro and in vivo activity of the compounds of formula I can be determined by various procedures known in the art, such as a test for their ability to inhibit the activity of the NK₁ agonist Substance P, an isolated hamster trachea NK₂ assay, a test of the effect of NK₁ antagonists on Substance P-induced airway microvascular leakage, measurement of NK₂ activity in vivo in guinea pigs, measurement of bronchoconstriction due to NKA, and neurokinin receptor binding assay(s). Typical procedures are described in WO96/34857, published Nov. 7, 1996. NK₃ activity is determined by following a procedure similar to that described in the literature, e.g., Molecular Pharmacol., 48 (1995), p. 711-716.

% Inhibition is the difference between the percent of maximum specific binding (MSB) and 100%. The percent of MSB is defined by the following equation, wherein "dpm" is disintegrations per minute: ##EQU1##

It will be recognized that compounds of formula I exhibit NK₁, NK₂ and/or NK₃ antagonist activity to varying degrees, e.g., certain compounds have strong NK₁ antagonist activity, but weaker NK₂ and NK₃ antagonist activity, while others are strong NK₂ antagonists, but weaker NK₁ and NK₃ antagonists. While compounds with approximate equipotency are preferred, it is also within the scope of this invention to use compounds of with unequal NK₁ /NK₂ /NK₃ antagonist activity when clinically appropriate.

Preferred compounds of the invention have a Ki≦10 nM for the NK₁ receptor. Also preferred are compounds of formula I having a Ki≦10 nM for the NK₂ receptor. Also preferred are compounds having a Ki≦10 nM for each of the NK₁ and NK₂ receptors. More preferred are compounds having a Ki≦2 nM for the NK₁ receptor and a Ki ≦2 nM for the NK₂ receptor. Compounds of the invention tested for NK₃ have Ki's in the range of 0.05 to 50 nM. The compound of Example 11, the only amide specifically disclosed in U.S. Pat. No. 5,696,267, has a Ki of 3.6 for the NK₁ receptor and a Ki of 9.2 for the NK₂ receptor.

Using the test procedures described above, the following 35 data (Ki) were obtained for exemplified compounds:

    ______________________________________                                                       Ki (NK.sub.1)                                                                           Ki (NK.sub.2)                                             Ex. (nM) (nM)                                                                ______________________________________                                         12E           0.4      0.2                                                       12F 0.6 0.8                                                                    12K 0.9 0.5                                                                    12L 0.5 0.9                                                                    13A 0.8 0.2                                                                    13B 0.9 0.3                                                                    13C 0.4 0.2                                                                    13D 1.1 0.7                                                                    13E 0.4 0.6                                                                    13F 0.2 0.3                                                                    13G 1.5 0.4                                                                    13H 2.6 0.4                                                                    14B 0.3 0.8                                                                    14D 0.3 0.2                                                                    14E 0.2 0.4                                                                    15A 0.2 0.7                                                                    15B 1.7 0.8                                                                    15D 0.4 0.5                                                                    15E 0.50 1.0                                                                   15F 0.4 0.8                                                                    15G 0.9 1.3                                                                    15H 0.2 0.4                                                                    15I 0.8 1.0                                                                    15J 0.7 4.3                                                                    15K 0.5 0.2                                                                    15L 1.0 1.4                                                                    15M 0.2 0.3                                                                    16A 0.2 0.7                                                                    17A 0.5 3.0                                                                    17E 0.7 0.7                                                                    17K 3.3 2.7                                                                    17S 0.8 0.8                                                                    18B 0.8 0.4                                                                    18D 0.4 0.4                                                                    18E 0.4 0.4                                                                    18J 0.3 3.4                                                                    18M 0.2 0.4                                                                    19C 0.2 0.2                                                                    19D 0.9 1.4                                                                    19E 0.6 1.8                                                                    21B 6 0.5                                                                      23 0.8 0.5                                                                     25 0.9 0.7                                                                     26 1.0 1.3                                                                     27 0.7 1.2                                                                     28B 0.8 1.0                                                                    28C 0.4 0.6                                                                    29B 5.2 1.3                                                                    29C 2.6 0.7                                                                    30A 1.4 1.9                                                                    31 0.4 1.8                                                                     35 0.6 0.3                                                                     36 0.8 1.0                                                                     37 0.7 0.7                                                                   ______________________________________                                     

We claim:
 1. A compound selected from the group consisting of ##STR230## and diastereomers, enantiomers, rotational isomers, E and Z isomers of the oxime, and pharmaceutically acceptable salts thereof.
 2. A compound of claim 1 selected from the group consisting of ##STR231##
 3. A compound of claim 2 selected from the group consisting of
 4. A compound of claim 1 which is or a diastereomer, enantiomer, rotational isomer, E and Z isomer of the oxime, or pharmaceutically acceptable salt thereof.
 5. A compound represented by the formula ##STR232## or a diastereomer, enantiomer, rotational isomer or E or Z isomer of the oxime, or a pharmaceutically acceptable salt, thereof, wherein:T is R² -phenyl or R³ -pyridyl; R¹ is H, methyl, ethyl, --CH₂ CN, --CH₂ C(O)NH₂, --(CH₂)₃ SO₃ H, --CH₂ C(O)NHCH₃, --CH₂ C(O)NHOH, --CH₂ C(O)NHOCH₃, --CH₂ C(O)NHCH₂ CN, --CH₂ F, --CH₂ C(O)NHCH₂ SO₃ H, ##STR233## R² is 2-3 substituents independently selected from the group consisting of chloro, methyl and methoxy; R³ is 2 to 3 substituents independently selected from the group consisting of chloro and methyl; R⁴ is methyl or ethyl; and Z is ##STR234##
 6. A compound of claim 5 wherein T is R² -substituted phenyl, wherein R² is two chloro substituents, two methyl substituents or two methoxy and one methyl substituent; R¹ is methyl, --CH₂ F, --CH₂ CN,
 7. A compound of claim 6 wherein R² is two chloro substituents, R¹ is methyl and Z is 